Fig 1: H2Bub redistribution upon UV irradiation is mediated by ATXN7L3 (A) Heatmaps depicting read densities for elongating RNAPII and H2Bub for genes larger than 60 kb (n = 1131) from TSS to TSS + 60 kb, aligned at individual (rows) active genes and ranked by increasing escape index (see Section 2) of the shCTL cells in non-irradiated conditions. (B) Average profiles illustrating the same annotation as (A). (C) Genome browser snapshots showing H2Bub (top) and RNAPII (bottom) in a representative gene in shCTL (top) and shATXN7L3 (bottom) cells in non-irradiated, 2 h and 24 h after UV irradiation conditions. (D) Density scatter plots illustrating DI for H2Bub in shCTL VS shATXN7L3 cells in non-irradiated conditions (left) depicting the accumulation of H2Bub signal in shATXN7L3 cells in proximal gene regions. A comparison of the change in DI (ΔDI = log2(+ UV/no UV)) 2 h post UV in the two cell lines (right), demonstrating a more extensive H2Bub redistribution in shATXN7L3 cells compared to shCTL cells upon UV. (E) Schematic illustrating the EI calculation (top) (EI, Section 2; comparison of the density of RNAPII at promoters (− 250 bp to + 100 bp around the TSS) to that in gene bodies (TSS + 100 bp to + 2 kb)). Density scatter plots showing the correlation between constitutive EI (NO UV) for RNAPII for all common active genes in shCTL VS shATXN7L3 (left) and a comparison of the change in EI (ΔEI = log2(+ UV/no UV)) 2 h post-UV (right). H2Bub and RNAPII samples shown in this figure are the result of two replicates merging.