Fig 1: Proteomic analysis of CRC-SCs-silenced cells discloses alterations in RNA metabolism, mitochondrial function and cytoskeleton remodeling.Semantic plot of the enriched biological processes (A). Proteomic validation through western blot of NUP21, filamin, villin, and SUCLG2 representative images (B) and quantification in CRC-SCs (C) Data represent the mean ± SD of at least 3 independent experiments. Representative images of transduced CRC-SC#1 and CRC-SC#2 stained with topro (blue) and in red filamin (D), LIMA (E), villin, and CDH17 (G) (scale bar 25 µm), IF quantification (F, H). Data represent the mean of at least 3 independent experiments ± SD. Student’s t-test was conducted (sh71 versus shNT): ***p < 0.001; ****p < 0.0001.