Fig 1: cGAS-STING pathway is essential for TMZ and anti-CD47 induced immune response.a Combination TMZ and anti-CD47 antibody treatment against murine GBM cells (GL261) increased the production of type I interferons in APCs. IFNA interferon a, IFNB interferon ß. n = 5, **p < 0.01, one-side ANOVA with Bonferroni post hoc correction. b Western blot showing combined TMZ and anti-CD47 antibody treatment resulted in the activation of cGAS-STING cytoplasmic DNA sensing pathways in BM APCs. c Combination TMZ and anti-CD47 antibody treatment promoted p65 expression and nuclear translocation in BM APCs that is dependent on STING. d STING activation is critical to the increased the production of NF-?B cytokine TNFa and IL1ß in APCs in the setting of combined TMZ and anti-CD47 antibody treatment. n = 6, **p < 0.01, unpaired Student’s t test. e Immunofluorescence staining of GL261 tumors implanted in WT and STING KO animals. Nuclear p-IRF3 (Ser 396) in CD45+ cells is indicative of STING signaling activation. f, g Quantification of CD45+ cell infiltration and percentage of p-IRF3 positive CD45+, C57BL/6, n = 7; knockout, n = 6. Unpaired Student’s t test. h GL261 tumor volume in C57BL/6 or STING KO mice at day 20 following control or combination TMZ + aCD47 treatment, n = 5/group. i T-cell infiltration in GL261 tumors in C57BL/6 or STING KO mice at day 20 following control or combination TMZ + aCD47 treatment measured by CD3+ cells in total DAPI count per field of view (FOV), C57BL/6, n = 9; knockout, n = 6 unpaired Student’s t test. Representative FOV for each group on the right. All error bars = mean ± standard deviation.
Supplier Page from Thermo Fisher Scientific for IRF3 Antibody