Fig 1: NAD + concentration and bio-synthesis changed in different subcellular compartments after DEHP treatment. Pregnant mice were administrated by DEHP (0, 5, 50 and 200 mg/kg/d) during GD 0–15. NAD+ content was measured in placental (A–D) nucleus, (E–H) cytoplasm, and (I–L) mitochondria, respectively. (M) NMNAT1, NMNAT2, and NMNAT3 representative images of Western blot. Quantifications of (N) NMNAT1, (O) NMNAT2, and (P) NMNAT3 expression in mice placenta based on band intensity were shown on the right side of the blots. All data are expressed as mean ± standard errors. (N = 6). * indicate a statistically significant difference (P < 0.05).