Fig 1: Neurochemical characterization of NTS leucine-sensing neurons. Representative images (a) and quantification (b–e) of multiplexed in situ hybridization against Prlh, Calcr, and Cfos in the dorsal vagal complex of mice after an injection of leucine into the NTS. Representative images of the colocalization of the Ha reporter from the polysynaptic retrograde virus PRV-Introvert and tyrosine hydroxylase (TH, f) or prolactin-releasing peptide (PrRP, g) by multiplexed immunofluorescent labeling in the NTS of the Agrp-ires-cre mice 96 h after PRV-Introvert delivery into the ARH. Scale bar: 200 μm. 4V: fourth ventricle, AP: area postrema, NTS: nucleus of the solitary tract. ∗p < 0.05 vs aCSF, ∗∗p < 0.01 vs aCSF, and ∗∗∗p < 0.001 vs aCSF. All the results are shown as means ± SEM.