Fig 1: Expression of Saa3 and Cxcl5 in the PS mesenchyme in Sox9CreER; Ctnnb1F/F mutant embryos. Immunofluorescence staining displayed that the expression levels of Saa3 and Cxcl5 in the wild-type PS mesenchyme was similar to that in Sox9CreER; Ctnnb1F/F mesenchyme. The nasal region of the PS mesenchyme distinguished by the white dashed lines. Error bars represent SD. ns: P>0.05. Scale bar, 50 µm. WT, wild type; PS, palatal shelf; DAPI, 4',6-diamidino-2-phenylindole; AN, nasal half of the anterior region; MN, nasal half of the middle region; PN, nasal half of the posterior region.
Fig 2: Comparison of significantly DEGs between NC and knockdown or overexpressed groups. (A) The number of DEGs in ß-catenin knockdown or overexpressed group compared to NC group. (B) Cluster heat map of DEGs between NC and knockdown or overexpressed groups. Red represents highly expressed DEGs, blue depicts poorly expressed genes. Highlighted with arrows are the four most differentially expressed genes, Saa3, Cxcl5, Cxcl1 and Steap4. NC, negative control group; KD, ß-catenin knockdown group; DEGs, differentially expressed genes.
Fig 3: Expression of Saa3 and Cxcl5 in the PS mesenchyme in Sox9CreER; Ctnnb1ex3f mutant embryos. Immunofluorescence staining displayed that the expression levels of Saa3 and Cxcl5 in the wild-type PS mesenchyme was similar to that in Sox9CreER; Ctnnb1ex3f mesenchyme. The nasal region of the PS mesenchyme distinguished by the white dashed lines. Error bars represent SD. ns: P>0.05. Scale bar, 50 µm. WT, wild type; PS, palatal shelf; DAPI, 4',6-diamidino-2-phenylindole; AN, nasal half of the anterior region; MN, nasal half of the middle region; PN, nasal half of the posterior region.
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