Fig 1: Functional identification of eccDNAs in colorectal cancer cell lines. a Heatmap of differential expression of genes REBB2, MYCBPAP, TP53I3, EGFR, SLC7A1, SLC29A1 in the samples. b-c DNA electrophoresis of inverse PCR for genes in Fig. 5a to verify eccDNA in HCT116 and Lovo respectively. G: genome DNA. LE: total DNA with linear DNA elimination. RCA: total DNA with linear DNA elimination after RCA. d Schematic representation of the sequencing results of PCR products of gene SLC7A1 in samples after RCA with matching the sequence in chr13. e-f Relative mRNA expression and protein levels of SLC7A1 and ß-actin in HCT116 transfected synthetic eccDNAs