Fig 2: CDK8, EEF1A1, or NTN1 knockdown repressed the proliferation and migration of antler cartilage cells. (A–C) Western blot analysis of the interference efficiency of CDK8, EEF1A1, or NTN1 siRNA in antler cartilage cells. The Western blot bands are representative images of three independent biological replicates, and fold-changes in densitometric values in three replicate experiments are presented as mean ± standard deviation. (D–F) The proliferation of antler cartilage cells transfected with si-CDK8 (D), si-EEF1A1 (E), si-NTN1 (F), or si-NC was detected by CCK-8 assay. (G–I) The effect of si-CDK8 (G), si-EEF1A1 (H), or si-NTN1 (I) on antler cartilage cell migration was measured by transwell migration assays. Scale bar represents 200 μm. si-NC, negative control siRNA. ** p < 0.01, *** p < 0.001.

Fig 3: MiR-PC-2869 blocks the expression of c-myc and cyclin D1 by targeting CDK8, EEF1A1, and NTN1. (A–D) Western blot analysis of MG63, SW1353, and antler cartilage cells transfected with miR-PC-2869 mimics (A), si-CDK8 (B), si-EEF1A1 (C), or si-NTN1 (D). The Western blot bands are representative images of three independent biological replicates, and fold-changes in densitometric values in three replicate experiments are presented as mean ± standard deviation. NC, negative control mimics. si-NC, negative control siRNA. CA, antler cartilage cells. (E) The schematic diagram illustrates the molecular mechanism through which miR-PC-2869 inhibits the proliferation and migration of osteosarcoma, chondrosarcoma, and antler cartilage cells.

Fig 4: Silencing of CDK8, EEF1A1, or NTN1 suppressed the proliferation, colony formation, and migration of chondrosarcoma cells. (A–C) Western blot analysis of SW1353 cells transfected with si-CDK8, si-EEF1A1, si-NTN1, or si-NC. The Western blot bands are representative images of three independent biological replicates, and fold-changes in densitometric values in three replicate experiments are presented as mean ± standard deviation. (D–L) The effects of CDK8, EEF1A1, or NTN1 knockdown on the proliferation (D–F), colony formation capacities (G–I), and migration (J–L) of SW1353 cells were assessed by CCK-8 assay, colony formation assay, and transwell migration assay, respectively. Scale bar represents 200 μm. si-NC, negative control siRNA. ** p < 0.01; *** p < 0.001.

Fig 5: Downregulation of CDK8, EEF1A1, or NTN1 inhibits proliferation, colony formation, and migration of osteosarcoma cells. (A–C) Western blot analysis of MG63 cells transfected with CDK8 (A), EEF1A1 (B), or NTN1 (C) siRNA. The Western blot bands are representative images of three independent biological replicates, and fold-changes in densitometric values in three replicate experiments are presented as mean ± standard deviation. (D–L) Effects of CDK8, EEF1A1, or NTN1 knockdown on the proliferation (D–F), colony formation capacities (G–I), and migration (J–L) of MG63 cells were evaluated using CCK-8 assay, colony formation assay, and transwell migration assay, respectively. Scale bar represents 200 μm. si-NC, negative control siRNA. * p < 0.05; ** p < 0.01; *** p < 0.001.