Fig 1: Colocalization of cytoplasmic/membrane CENPB and MHC Class II molecules in lcSSc skin fibroblasts of patients with ACAs.We performed IF to visualize CENPB (green) and the expression of the MHC class II molecules DRB1 (beta 1 chain: purple) and DRB5 (beta 5 chain: red) in SSc and healthy skin fibroblasts. Nuclei were counterstained with DAPI (Blue). Shown are representative pictures of at least 10 micrographs. Arrows indicate colocalization of cytoplasmic/membrane CENPB and both MHCII regions in lcSSc patients (025 and 111), who have ACAs. No colocalization was visualized in dcSSc skin fibroblasts (043 and 116). The scale bar is shown at the bottom right.