Fig 1: Spontaneous neural differentiation from generated NPCs. (A) NPCs were spontaneously differentiated into neurons and astrocytes, represented by TUBB3 and GFAP staining, respectively. Scale bar, 50 µm. (B) Synapse formation by SYN1 staining of differentiated neurons at 30 dpi. Scale bar: 25 µm. (C) Complicated morphology of differentiated neurons at 30 dpi. Scale bar: 20 µm. (D,E) Neural subtypes differentiated from SB/LDN NPCs. Scale bar: 50 µm. (F) Repetitive action potential waveforms recorded by whole-cell patch-clamp recording. (G) Representative waveform of inward and outward currents. (H) A zoomed-in view of inward currents, resembling sodium currents. (F–H) Adapted from our previous results [9].