Fig 1: Inhibition of H3K9me3 induces cytosolic localization of histone H3. A) SETDB1 was knocked out in U2OS cells and histone H3 were analyzed by confocal microscopy. Scale bar, 5 µm. B) GFP-LC3B-U2OS Cells were treated with 5 µm BIX-01294 for 8 h and H3 was studied with confocal microscopy. Three different H3 antibodies were used. Scale bar, 5 µm. C) HA-SETDB1, Flag-G9A, and GFP-SUV39H1 were transfected into U2OS cells and treated with 5 µm BIX-01294 for 8 h. The indicated proteins were imaged with confocal microscope. Scale bar, 10 µm. D) DNA was stained by anti-DNA antibody and studied with confocal microscopy. Mitochondria were labeled with Mito-tracker (Invitrogen, M7521). Cells were treated with 5 µm BIX-01294 for 8 h. E) GFP-LC3-U2OS cell were treated with 5 µm BIX-01294 for 12 h. Histone H4 was imaged with confocal microscopy. Scale bar, 5 µm.