Anti-Collagen VI antibody ab6588 from Abcam

Product Specs
ItemAnti-Collagen VI antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab6588
Antibody TypePrimary
Quantity100 UG
ApplicationsWestern blot
ClonalityPolyclonal
HostRabbit
IsotypeIgG
Conjugate/TagUnconjugated
FormatImmunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities
Concentration1 mg/ml
Gene NameCOL6A1
ReactivityHuman
TargetCollagen VI

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Abcam

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Pirfenidone increases transverse tubule length in the infarcted rat myocardiumInterface FocusDecember 6, 2023Hussam Moammer, Jizhong Bai, Timothy L. M. Jones, Marie Ward, Caroyln Barrett, David J. Crossmanagitation, and incubated overnight at 4°C with a rabbit anti-collagen VI primary antibody (1 : 500, ab6588, Abcam) in the same blocking buffer. Following washes with Tris-buffered saline with 0.01% Tween 20

(2) Longitudinal assessment of skeletal muscle functional mechanics in the DE50-MD dog model of Duchenne muscular dystrophyDis Model MechDecember 5, 2023Dominique O. Riddell, John C. W. Hildyard, Rachel C. M. Harron, Frances Taylor-Brown, Joe N. Kornegay, Dominic J. Wells, Richard J. Piercymin in three changes of PBS. Sections were incubated with rabbit collagen VI antibody (1:1000, Abcam, ab6588) for 60 min at room temperature. Slides were washed again over 5 min in three changes of PBS. Sections

(3) Mechanical loading due to muscle movement regulates establishment of the collagen network in the developing murine skeletonRoyal Society Open ScienceOctober 18, 2023Saima Ahmed, Andrew V. Rogers, Niamh C. NowlanUK",MAB8887 collagen III,"Abcam, UK",ab7778 collagen V,"Abcam, UK",ab7046 collagen VI,"Abcam, UK",ab6588 collagen X,"Abcam, UK",ab58632 collagen XI,"Invitrogen, UK",PA5-77258 goat anti-rabbit (Cy3®),"Abcam,

(4) Immunohistochemical Analysis of Knee Chondral Defect Repair after Autologous Particulated Cartilage and Platelet-Rich Plasma Treatment in SheepInternational Journal of Molecular SciencesOctober 13, 2023Lourdes Alcaide-Ruggiero, Verónica Molina-Hernández, Juan Morgaz, J. Andrés Fernández-Sarmiento, María M. Granados, Rocío Navarrete-Calvo, José Pérez, Setefilla Quirós-Carmona, José M. Carrillo, Ramón Cugat, et al.polyclonal,1/200,Rabbit Collagen V,Abcam φ,ab7046,Rabbit polyclonal,1/200,Rabbit Collagen VI,Abcam φ,ab6588,Rabbit polyclonal,1/1000,Rabbit Collagen IX,Abcam φ,ab134568,Rabbit polyclonal,1/200,Rabbit Collagen

(5) Hyperplastic Human Macromass Cartilage for Joint RegenerationAdvanced ScienceSeptember 1, 2023Ya Wen, Yishan Chen, Weiliang Wu, Hong Zhang, Zhi Peng, Xudong Yao, Xianzhu Zhang, Wei Jiang, Youguo Liao, Yuan Xie, et al.antibodies against COL2A1 (1:100, Santa Cruz, sc‐52658), ACAN (1:200, Abcam, ab36861), COL6 (1:250, Abcam, ab6588), PRG4 (1:100, Abcam, ab28484), and COL1 (1:200, Affinity, AF7001) overnight at 4 °C. Then, sections

(6) Effects of Aging on Collagen in the Skeletal Muscle of MiceInternational Journal of Molecular SciencesAugust 23, 2023Yuji Kanazawa, Ryo Miyachi, Takashi Higuchi, Hiaki Satoincubated with rabbit polyclonal anti-collagen I, III, IV, or VI antibodies (ab21286, ab7778, ab6586, ab6588; Abcam, Cambridge, MA, USA) diluted to 1:500 in PBS containing 0.3% Triton X-100 at 4 °C for 24 h. Subsequently,

(7) Dysregulation of TSP2-Rac1-WAVE2 axis in diabetic cells leads to cytoskeletal disorganization, increased cell stiffness, and dysfunctionScientific ReportsDecember 28, 2022Hao Xing, Yaqing Huang, Britta H. Kunkemoeller, Peter J. Dahl, Ohvia Muraleetharan, Nikhil S. Malvankar, Michael P. Murrell, Themis R. Kyriakidesab2413), anti-mouse collagen type I (1:1000, EMD Millipore, AB765P), anti-collagen VI (1:100, Abcam, ab6588), anti-WAVE2 (1:50, Cell Signaling, ab3569S), anti-ARPC2 (1:50, Abcam, ab11798), anti-Rac1 (1:100, Abcam,

(8) Altered electrical properties in skeletal muscle of mice with glycogen storage disease type IIScientific ReportsDecember 1, 2022Janice A. Nagy, Carson Semple, Daniela Riveros, Benjamin Sanchez, Seward B. Rutkoveby light microscopy. Additional paraffin sections were stained with anti–collagen VI antibody (Abcam ab6588) to identify the myocyte cell membranes and 4',6-diamidino-2-phenylindole (DAPI) to detect nuclei. The

(9) A loss-of-function cysteine mutant in fibulin-3 (EFEMP1) forms aberrant extracellular disulfide-linked homodimers and alters extracellular matrix compositionHuman mutationDecember 1, 2022Woodard DR, Daniel S, Nakahara E, Abbas A, DiCesare SM, Collier GE, Hulleman JD.serum, 0.1% BSA, 0.1% Triton-X dissolved in PBS. The primary antibody (rabbit antiColVI, 1:200; Abcam #ab6588) was incubated at 4°C overnight with gentle rocking. Membranes were washed 2x followed by secondary

(10) The Effect of TGFβ1 in Adipocyte on Inflammatory and Fibrotic Markers at Different Stages of Adipocyte DifferentiationPathophysiologyNovember 23, 2022Babu Raja Maharjan, Susan V. McLennan, Stephen M. Twigg, Paul F. Williams(Roche, Rotkreuz, Switzerland), as explained earlier [11]. Anti-collagen VI antibody (catalog number ab6588, Abcam, Cambridge, UK) and anti-rabbit IgG for the secondary antibody (catalog number S9169, Sigma®)

(11) Histochemical Comparison of Human and Rat Lacrimal Glands: Implications for Bio-Engineering StudiesTranslational vision science & technologyNovember 1, 2022Wood JPM, Chidlow G, Halliday LA, Casson RJ, Selva D, Sun M.IV,Rabbit,1:1000,Abcam,ab6586 Collagen V,Goat,1:1000,Chemicon,ab781 Collagen VI,Rabbit,1:1000,Abcam,ab6588 Cytokeratin-8,Mouse,1:500,Dako,*35bH11 Cytokeratin-18,Mouse,1:1000,GeneTex,*C-04 eNOS,Mouse,1:1000,BD

(12) Decellularized Pancreatic Tail as Matrix for Pancreatic Islet Transplantation into the Greater Omentum in RatsJournal of functional biomaterialsSeptember 30, 2022Berkova Z, Zacharovova K, Patikova A, Leontovyc I, Hladikova Z, Cerveny D, Tihlarikova E, Nedela V, Girman P, Jirak D, et al.1:100; Abcam, Cambridge, UK), anti-collagen IV (ab6586, 1:100; Abcam, Cambridge, UK), anti-collagen VI (ab6588, 1:100; Abcam, Cambridge, UK), anti-entactin/NID (ab14511, 1:100; Abcam, Cambridge, UK), and anti-collagen

(13) Development of 2-D and 3-D culture platforms derived from decellularized nucleus pulposusFrontiers in Bioengineering and BiotechnologySeptember 27, 2022Marco A. Herrera Quijano, Nadia Sharma, Pascal Morissette Martin, Cheryle A. SÃ©guin, Lauren E. Flynncollagen type II (1:200, ab34712, Abcam), collagen type V (1:300, ab7046, Abcam), collagen type VI (1:300, ab6588, Abcam), fibronectin (1:150, ab23750, Abcam), laminin (1:200, ab11575, Abcam), or keratan sulphate (1:200,

(14) In Vitro and Ectopic In Vivo Studies toward the Utilization of Rapidly Isolated Human Nasal Chondrocytes for Single-Stage Arthroscopic Cartilage Regeneration TherapyInternational journal of molecular sciencesJune 21, 2022Lehoczky G, Trofin RE, Vallmajo-Martin Q, Chawla S, Pelttari K, Mumme M, Haug M, Egloff C, Jakob M, Ehrbar M, et al.with 5% bovine serum albumin, and rabbit anti collagen VI (human and mouse specific, Abcam Ref. No. ab6588, 1:500 dilution) primary antibody was applied. Detection of immune-binding was performed with Alexa-Fluor-488-conjugated

(15) Multi-Scale Analysis of the Composition, Structure, and Function of Decellularized Extracellular Matrix for Human Skin and Wound Healing ModelsBiomoleculesJune 16, 2022Atiya M. Sarmin et al.ab90395, Abcam, Cambridge, UK), 1:1000 collagen III (mouse, ab7778, Abcam), or 1:1000 collagen VI (mouse, ab6588, Abcam), diluted in 5% milk TBS-T overnight at 4 °C. The membranes were washed three times in TBS-T,

(16) Nerve bundle formation during the promotion of peripheral nerve regeneration: collagen VI-neural cell adhesion molecule 1 interactionNeural regeneration researchMay 1, 2022Sun JH, Huang M, Fang Z, Li TX, Wu TT, Chen Y, Quan DP, Xu YY, Wang YM, Yang Y, et al.instructions. In brief, 5 μg anti-NCAM1 antibody (Abcam, Cat# ab220360) or 5 μg anti-COL6 antibody (Abcam, Cat# ab6588) was incubated with 1.5 mg Dynabeads in the Ab Binding & Washing Buffer to form the Dynabeads-Ab complex.

(17) Effective high-throughput isolation of enriched platelets and circulating pro-angiogenic cells to accelerate skin-wound healingCellular and Molecular Life SciencesMay 1, 2022Silvia Erratico et al.BD Biosciences-Pharmingen, San Diego, California, USA), rabbit polyclonal antibody anti-collagen VI (1:250, ab6588, Abcam, UK), mouse monoclonal antibody anti-alpha SMA (1:50, A2547, Sigma-Aldrich Inc., St. Louis, MO,

(18) Exercise training remodels subcutaneous adipose tissue in adults with obesity even without weight lossThe Journal of physiologyMay 1, 2022Ahn C, Ryan BJ, Schleh MW, Varshney P, Ludzki AC, Gillen JB, Van Pelt DW, Pitchford LM, Howton SM, Rode T, et al.Sigma Aldrich), Collagen type 5a3 (Col5a3, #LS-C353420, LifeSpan BioSciences), Collagen type 6 (Col6, # ab6588, Abcam). Secondary antibodies were AlexaFlour 488 (# A11008, A21422, Thermofisher Scientific) and 555

(19) Ghrelin Represses Thymic Stromal Lymphopoietin Gene Expression through Activation of Glucocorticoid Receptor and Protein Kinase C Delta in Inflamed Skin KeratinocytesInternational Journal of Molecular SciencesApril 2, 2022Hayan Jeong, Hyo-Jin Chong, Jangho So, Yejin Jo, Tae-Young Yune, Bong-Gun Ju(720078, Invitrogen, Waltham, MA, USA), anti-loricrin (ab24722, Abcam, Cambridge, UK), anti-collagen VI (ab6588, Abcam, Cambridge, UK), anti-p300 (SC-584, Santa Cruz Biotechnology, Dallas, TX, USA), anti-phospho

(20) Metabolomic Profiling and Mechanotransduction of Single Chondrocytes Encapsulated in Alginate MicrogelsCellsMarch 5, 2022Jacob P. Fredrikson, Priyanka P. Brahmachary, Ayten E. Erdoğan, Zachary K. Archambault, James N. Wilking, Ronald K. June, Connie B. Changthen incubated with the primary antibody to collagen VI (Rabbit Polyclonal Anti-collagen VI antibody, ab6588 from Abcam, Boston, MA, USA) in Triton X-100 (0.1% v/v)–Bovine Serum Albumin (1% v/v)–PBS (1X) for 1

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Anti-Collagen VI antibody from Abcam

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Anti-Collagen VI antibody from Abcam

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