Fig 1: Intrinsic Rig-I deficiency boosts antitumor activity of adoptively transferred CD8+ T cells.(A and B) 2 × 106 Naive CD8+ T cells from spleens of Rig-I+/+ or Rig-I–/– mice were i.v. transfused into Rag1–/– mice. Saline was used as a vehicle control. 2 × 105 MC38 cells were s.c. inoculated 1 day after T cell injection and tumor volume was measured on days 10, 13, 15, 19, and 21 (n = 6 per group). Experimental design (A) and tumor growth curve (B) are shown. (C) CD45+CD8+ cell number was counted using flow cytometry. (D and E) Annexin V, IFN-γ, and CD107a were detected by flow cytometry. The percentage of Annexin V+ cells (D) and IFN-γ+ or CD107a+ cells (E) in CD45+CD8+ cells are shown. (F and G) Immunofluorescence staining of tumor tissue was performed to detect the nuclei (DAPI; blue), CD8 (green) and granzyme B (red). 10 random fields of view for each group were analyzed. Representative images (F) and combined plots (G) of the percentages of GZMB+CD8+ T cells were shown. Data are representative of 2 independent experiments and expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, by 2-way ANOVA (B) or unpaired Student’s t test (C–E and G).