Fig 2: Pyroptosis is activated in the lung tissues of mice infected with A. fumigatus after irradiation. a Representative western blot images showing changes in the levels of caspase 1-p20, gasdermin D (GSDMD-N), interleukin 18 (IL-18), IL-1ß cleaved, b–e and their statistical results. f–i mRNA expression of ASC, NLRP3, NLRC4, and AIM2. j, k Secretion of IL-18 and IL-1ß in BALF. Data are shown as the mean ± standard deviation (n = 8). *P < 0.05, **P < 0.01 versus the control group

Fig 3: Inflammasome signaling plays important roles in cancer cells and TAM interaction. (A) Immunohistochemistry and quantitative correlation analyses of AIM2 and NLRP3 expression and IBA-1+ TAM infiltration in GBM patients. Scale bar: 25 µm. (B and C) Western blotting analysis of the inflammasome (NLRP3 and AIM2), C-Caspase 1 (cleaved caspase 1), and PD-L1 expression in THP-1 co-cultured GBM and BRCA cells, pre-treated with MB (B) or CASP1 targeted siRNA (C). (D and E) Migration analysis of THP-1, co-cultured with cancer cells that were pre-treated with MB (D) or CASP1 targeted siRNA (E). Scale bar: 25 µm. (F and G) Western blotting analysis of PD-L1 expression in THP-1 which was co-cultured with cancer cells pre-treated with MB (F) or CASP1 targeted siRNAs (G). **p < 0.01, ***p < 0.001, ****p < 0.0001.

Fig 4: PKIB and AIM2 involved in survival of osteosarcoma. (A) Q-PCR results to show the expression level of AIM2 and PKIB genes. (B) Multivariate Cox regression analyses of AIM2 and PKIB genes with overall survival. (C) Representative images of western blot to show the protein expression level of AIM2 and PKIB. (D) Bar graphs showed the normalized protein expression intensity of AIM2 and PKIB. (E) The distribution of AIM2 and PKIB DNA methylation level. n>3, Error bars: Mean±SD. ***P<0.001.

Fig 5: Nomogram for overall survival prediction in osteosarcoma patients. (A) The nomogram consists of risk score based on the AIM2/PKIB expression profile. (B) The ROC curves of the risk subgroups by the tertiles of total points derived from the nomogram.