Anti-BrdU antibody, Mouse monoclonal B8434 from MilliporeSigma

Product Specs
ItemAnti-BrdU antibody, Mouse monoclonal
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberB8434
Quantity100µL, 200µL, 25µL
Applicationsimmunocytochemistry, immunohistochemistry (formalin-fixed, paraffin-embedded sections)
Conjugate/TagUnconjugated
Reactivitywide range
TargetBrdU
IsotypeIgG1
CloneBU-33
Hostmouse
ClonalityMonoclonal
Concentration~1.5 mg/mL

Add to Compare List

MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

Company Profile

Website: www.SigmaAldrich.com/

(1) Ribosome biosynthesis and Hedgehog activity are cooperative actionable signaling mechanisms in breast cancer following radiotherapynpj Precision OncologyJune 28, 2023Brandon J. Metge, Heba A. Alsheikh, Dongquan Chen, Amr R. Elhamamsy, Dominique C. Hinshaw, Bo-Ruei Chen, Barry P. Sleckman, Rajeev S. Samant, Lalita A. Shevde0.3% Triton X-100 for 15 min, blocked in 5% BSA, and incubated with anti-Brdu 1:400 (Millipore Sigma, B8434) and anti-Fibrillarin 1:400 (Abcam, Cambridge UK, ab166630) overnight at 4 °C. Coverslips were incubated

(2) CDT1 is the major functional regulatory subunit of the pre‐replication complex in zygotesCell ProliferationMarch 1, 2023Chao Li, Yong‐Peng Tan, Xue‐Shan Ma, Zhen‐Bo Wang, Tie‐Gang Meng, Qing‐Yuan Sunzygotes were blocked in BSA for 1 h at room temperature, then incubated with anti‐BrdU antibody (1:1000;B8434; Sigma‐Aldrich) at 4°C overnight, washed with PBST every 10 min for four times, and finally incubated

(3) Clearance of p16Ink4a-positive cells in a mouse transgenic model does not change β-cell mass and has limited effects on their proliferative capacityAging (Albany NY)January 12, 2023Nadine Bahour, Lucia Bleichmar, Cristian Abarca, Emeline Wilmann, Stephanie Sanjines, Cristina Aguayo-Mazzucatoab195956,1:400 Anti-FLAG,Rabbit,R&D; MAB8529,1:250 Anti-FLAG,Mouse,Sigma Aldrich; F1804,1:500 Anti-BrdU,Mouse,Sigma Aldrich; B8434,1:50 488 anti-Guinea Pig IgG,Donkey,Jackson ImmunoResearch Laboratories; 706-545-148,1:200 594 anti-Rabbit

(4) Accumulation of DNA damage alters microRNA gene transcription in Arabidopsis thalianaBMC Plant BiologyDecember 12, 2022Juan Du, Yang Liu, Lu Lu, Jianfei Shi, Longqian Xu, Qi Li, Xiaofei Cheng, Jinfeng Chen, Xiaoming Zhang0.05% Tween-20, 37.5 mM NaCl, and 1 mM EDTA) and incubate with 2 μg anti-BrdU antibody (Sigma-Aldrich, B8434) at 4 °C for 2 h and then subjected to immunoprecipitation for 1 h with Dynabeads protein G (Invitrogen,

(5) 4-phenylbutyric acid-Identity crisis; can it act as a translation inhibitor?Aging cellDecember 1, 2022Stein D, Slobodnik Z, Tam B, Einav M, Akabayov B, Berstein S, Toiber D.and incubated overnight in 4°C with anti‐BrdU (diluted 1:300 in blocking solution; catalog number: B8434, Sigma‐Aldrich). On the following day, each well was washed 3 times with washing solution, then incubated

(6) Characterization of hemocytes and hematopoietic cells of a freshwater crayfish based on single-cell transcriptome analysisiScienceAugust 19, 2022Irene Söderhäll, Erik Fasterius, Charlotta Ekblom, Kenneth SöderhällRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Anti-BrdU mouse monoclonal,Sigma,"Cat# B8434, RRID:AB_476811" Anti-mouse-FITC,Sigma,"Cat# F3008, RRID:AB_259513" ,, "Chemicals, peptides, and

(7) Chromosome silencing in vitro reveals trisomy 21 causes cell-autonomous deficits in angiogenesis and early dysregulation in Notch signalingCell reportsAugust 9, 2022Moon JE, Lawrence JB.RESOURCE,SOURCE,IDENTIFIER ,, Antibodies,Antibodies,Antibodies ,, anti-BrdU,Sigma-Aldrich,Cat#: B8434; RRID:AB_476811 anti-CD31 (rabbit polyclonal),Abcam,ab28364 anti-H2Ak119Ub (rabbit monoclonal),Cell Signaling

(8) Hypothalamic Nesfatin-1 Resistance May Underlie the Development of Type 2 Diabetes Mellitus in Maternally Undernourished Non-obese RatsFrontiers in NeuroscienceMarch 21, 2022Máté Durst, Katalin Könczöl, Klementina Ocskay, Klaudia Sípos, Péter Várnai, Anett Szilvásy-Szabó, Csaba Fekete, Zsuzsanna E. Tóthperform antigen retrieval. The following primary antibodies were used: mouse anti-BrDU (1:1,000, Cat. # B8434, Merck) and rabbit anti-nesfatin-1 (1:6,000, Cat. # H-003-22, Phoenix, recognizes also NUCB2 protein).

(9) Oxidative stress induces Z-DNA-binding protein 1-dependent activation of microglia via mtDNA released from retinal pigment epithelial cellsThe Journal of biological chemistryJanuary 1, 2022Saada J, McAuley RJ, Marcatti M, Tang TZ, Motamedi M, Szczesny B.STING (Cell Signaling #13647), IRF3 (abcam#124399), TBK1 (Thermo Scientific #108A429), BrDU (Sigma #B8434). Images were captured using a Nikon eclipse 80i inverted fluorescent microscope with Photometric CoolSNAP

(10) Glia from the central and peripheral nervous system are differentially affected by paclitaxel chemotherapy via modulating their neuroinflammatory and neuroregenerative propertiesFrontiers in pharmacologyJanuary 1, 2022Klein I, Boenert J, Lange F, Christensen B, Wassermann MK, Wiesen MHJ, Olschewski DN, Rabenstein M, Müller C, Lehmann HC, et al.proliferation rate via BrdU-incorporation, astrocytes were stained with anti-BrdU (1:200; Sigma-Aldrich Cat# B8434, RRID:AB_476811). For visualization of primary antibodies, fluorescein-labelled anti-mouse immunoglobulin

(11) LncRNA NEAT1 Recruits SFPQ to Regulate MITF Splicing and Control RPE Cell ProliferationInvestigative Opthalmology & Visual ScienceNovember 17, 2021Xiaojuan Hu et al.analysis, cells were cultured in complete medium containing 20 µm BrdU for 2 hours. Anti-BrdU (1:200; b8434; Sigma-Aldrich, St. Louis, MO, USA) was used for immunostaining. Staining was indicated by appropriate

(12) Involvement of Epidermis Cell Proliferation in Defense Against Beauveria bassiana InfectionFrontiers in ImmunologySeptember 16, 2021Wuren Huang et al.DNA duplication in epidermis cells for three hours (17). The primary antibody against BrdU (Sigma, B8434; 1:1000) was utilized to label cells that had incorporated BrdU as described in detail (17, 18). The

(13) Mutation-induced DNMT1 cleavage drives neurodegenerative diseaseScience AdvancesSeptember 3, 2021Wencai Wang, Xingsen Zhao, Yanjiao Shao, Xiaoya Duan, Yaling Wang, Jialun Li, Jiwen Li, Dali Li, Xuekun Li, Jiemin Wongaccording to the manufacturer’s directions. For BrdU, DCX, and NeuN stainings, BrdU (Sigma-Aldrich, B8434), DCX (Cell Signaling Technology, no. 4604), and NeuN (Millipore, MAB377) antibodies were used, respectively.

(14) A Chinese hamster transcription start site atlas that enables targeted editing of CHO cellsNAR Genomics and BioinformaticsJuly 13, 2021Isaac Shamie et al.libraries generated exactly as described in (31). BrU enrichment was performed using a BrdU Antibody (Sigma B8434-200 μl Mouse monoclonal BU-33) coupled to Protein G (Dynal 1004D) beads. For each sample, 3 × 20 μl

(15) Protocol for analysis of G2/M DNA synthesis in human cellsSTAR ProtocolsJune 18, 2021Jianming Wang, Marco Saponaroanti-phospho-histone H3 (Ser10),Cell Signaling Technology,Cat#3377S; RRID: AB_1549592 Mouse monoclonal anti-BrdU,Sigma-Aldrich,Cat#B8434; RRID: AB_476811 Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L),Thermo Fisher Scientific,Cat#A-11070;

(16) The Peptide TAT-I24 with Antiviral Activity against DNA Viruses Binds Double-Stranded DNA with High AffinityBiologicsJune 10, 2021Hanna Harant, Siegfried Höfinger, Franz Kricek, Christine Ruf, Zsolt Ruzsics, Hartmut Hengel, Ivan J. D. Lindley(TBE) buffer for neutralization. Staining was performed with a mouse monoclonal anti-BrdU antibody (#B8434; Sigma-Aldrich Schnelldorf, Germany) 1:250 diluted in PBS + 1% FCS overnight at 4 ◦ C and a goat anti-mouse

(17) Reduced Granule Cell Proliferation and Molecular Dysregulation in the Cerebellum of Lysosomal Acid Phosphatase 2 (ACP2) Mutant MiceInternational Journal of Molecular SciencesMarch 15, 2021Xiaodan Jiao, Maryam Rahimi Balaei, Ejlal Abu-El-Rub, Filippo Casoni, Hassan Pezeshgi Modarres, Sanjiv Dhingra, Jiming Kong, Giacomo G. Consalez, Hassan Marzbanand cryostat cerebellar sections were subjected to IHC using mouse monoclonal anti-BrdU (Sigma, Cat# B8434, 1:500). 4.6. Western Blotting Cerebellum samples were collected from nax mutants and wt littermates

(18) Persistence of RNA transcription during DNA replication delays duplication of transcription start sites until G2/MCell ReportsFebruary 16, 2021Jianming Wang, Patricia Rojas, Jingwen Mao, Martina Mustè Sadurnì, Olivia Garnier, Songshu Xiao, Martin R. Higgs, Paloma Garcia, Marco SaponaroH3,Cell Signaling Technology,Cat#9715S; RRID: AB_331563 Mouse monoclonal anti-BrdU,Sigma-Aldrich,Cat#B8434; RRID: AB_476811 Mouse monoclonal anti-Tubulin,The Francis Crick Institute Core Facility,Tat-1 Mouse

(19) MAPK4 promotes prostate cancer by concerted activation of androgen receptor and AKTThe Journal of clinical investigationFebruary 15, 2021Shen T, Wang W, Zhou W, Coleman I, Cai Q, Dong B, Ittmann MM, Creighton CJ, Bian Y, Meng Y, et al.(catalog R0908), BrdU (catalog B9285), and antibodies against β-ACTIN (catalog A1978), BrdU (catalog B8434), and FLAG (catalog F3165) were purchased from MilliporeSigma. Enzalutamide (MDV3100, catalog S1250)

(20) Modulating hESC-derived cardiomyocyte and endothelial cell function with triple-helical peptides for heart tissue engineeringBiomaterialsFebruary 1, 2021Colzani M, Malcor JD, Hunter EJ, Bayraktar S, Polkinghorne M, Krieg T, Cameron R, Best S, Farndale RW, Sinha S.polyclonal anti CD31 (I),Novus biologicals,NB100-2284 Mouse monoclonal Anti-BrdU antibody (I),Sigma,B8434 Mouse monoclonat Anti-Connexin-43 (I),Millipore,MAB3067 Donkey anti Rabbit Alexa 488 (II),Life Technologies,R37118 Donkey

Be the First to Write a Review!

Anti-BrdU antibody, Mouse monoclonal from MilliporeSigma

MilliporeSigma

Citations (212)

Reviews

Anti-BrdU antibody, Mouse monoclonal from MilliporeSigma

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map