Monoclonal Anti-Myosin (Skeletal, Fast)−Alkaline Phosphatase antibody produced in mouse A4335 from MilliporeSigma

Product Specs
ItemMonoclonal Anti-Myosin (Skeletal, Fast)−Alkaline Phosphatase antibody produced in mouse
CompanyMilliporeSigma
Price Supplier Page View Company Product Page
Catalog NumberA4335
Conjugate/Tagalkaline phosphatase conjugate
Reactivityfeline, rat, bovine, chicken, human, rabbit, mouse, guinea pig
TargetMYH2; rat Myh2; rat Myh1; mouse Myh2; mouse Myh1; human MYH1
CloneMY-32
Hostmouse
Antibody Typeprimary antibodies
ApplicationsImmunofluorescence (IF)
Clonalitymonoclonal
NCBI Full Gene Namemyosin, heavy polypeptide 2, skeletal muscle, adult, myosin heavy chain 2, myosin heavy chain 1, myosin, heavy polypeptide 1, skeletal muscle, adult
NCBI Gene AliasesHEL71, MyHC-IId/x, MYHC-IIX, IId, MyHC-IIa, Myhs-f1, MHC2X/D, MYPOP, MYH2A, Myhsf1, Myhsf2, MHC-2X/D, MyHC-IIA, MdMs, MYHas8, MyHC-IIx/d, Myh2a, MHC2A, MYHC, MyHC-2x, MyHC-2X/D, A530084A17Rik, MYHSA1, MYHSA2, IBM3, Myh1, Myhs-f, MyHC-2A, MYHa, IId/x, Myhs-f2

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MilliporeSigma

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Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

Company Profile

Website: www.SigmaAldrich.com/

(1) Caloric Restriction Combined with Immobilization as Translational Model for Sarcopenia Expressing Key-Pathways of Human PathologyAging and diseaseJune 1, 2023Jelle C.B.C de Jong, Martien P.M Caspers, Nanda Keijzer, Nicole Worms, Joline Attema, Christa de Ruiter, Serene Lek, Arie G Nieuwenhuizen, Jaap Keijer, Aswin L Menke, et al.immunolabeled by a 1-hour incubation in 1/50 alkaline phosphatase conjugated mouse anti-fast myosin antibody (A4335; Sigma-Aldrich) and developed using a red alkaline phosphatase substrate kit (SK5100; Vector). For negative

(2) Neuromuscular Adaptations Following Training and Protein Supplementation in a Group of Trained WeightliftersSports (Basel)April 19, 2018Christopher Taber, Kevin Carroll, Brad DeWeese, Kimitake Sato, Charles Stuart, Mary Howell, Kenton Hall, Caleb Bazyler, Michael StoneVector Laboratories (Burlingame, CA, USA). Monoclonal anti-myosin (skeletal-fast) alkaline phosphatase (A4335) was purchased from Sigma (St. Louis, MO, USA). Monoclonal mouse anti-slow muscle myosin (MAB1628) was

(3) Pre-Training Muscle Characteristics of Subjects Who Are Obese Determine How Well Exercise Training Will Improve Their Insulin ResponsivenessJournal of Strength and Conditioning ResearchMarch 1, 2017Charles A. Stuart, Michelle L. Lee, Mark A. South, Mary E.A. Howell, Brian M. Cartwright, Michael W. Ramsey, Michael H. Stonewas a bright field staining method using labeled fast and slow myosin antibodies from Sigma-Aldrich (A4335) and Fisher (50-174-899, NC9996165, and NC9799788). All sections were coded and then quantified independently

(4) Myosin content of individual human muscle fibers isolated by laser capture microdissectionAmerican Journal of Physiology-Cell PhysiologyMarch 1, 2016Charles A. Stuart, William L. Stone, Mary E. A. Howell, Marianne F. Brannon, H. Kenton Hall, Andrew L. Gibson, Michael H. Stone(SAB1410809) from Sigma-Aldrich (St. Louis, MO); monoclonal anti-myosin (skeletal-fast)-alkaline phosphatase (A4335) from Sigma; peroxidase-conjugated rabbit anti-mouse antibodies from Jackson Immunoresearch Labs (West

(5) Inhibition of COX1/2 alters the host response and reduces ECM scaffold mediated constructive tissue remodeling in a rodent model of skeletal muscle injuryActa BiomaterialiaFebruary 1, 2016Christopher L. Dearth, Peter F. Slivka, Scott A. Stewart, Timothy J. Keane, Justin K. Tay, Ricardo Londono, Qingnian Goh, Francis X. Pizza, Stephen F. Badylakincubation in alkaline phosphatase conjugated mouse anti-fast myosin heavy chain (1:200, clone MY-32, A4335, Sigma) for 1 hour. Color was developed by staining with alkaline phosphatase (Red Alkaline Phosphatase

(6) Variations in the efficiency of lineage marking and ablation confound distinctions between myogenic cell populationsDevelopmental cellDecember 8, 2014Glenda Comai, Ramkumar Sambasivan, Swetha Gopalakrishnan, Shahragim Tajbakhsh

(7) Uniaxial cyclic strain enhances adipose-derived stem cell fusion with skeletal myocytesBiochemical and biophysical research communicationsJuly 25, 2014Jens Isak Andersen, Morten Juhl, Thøger Nielsen, Jeppe Emmersen, Trine Fink, Vladimir Zachar, Cristian Pablo Pennisi

(8) Effect of tolerating macronutrient deficit on the development of intensive-care unit acquired weakness: a subanalysis of the EPaNIC trialThe Lancet Respiratory MedicineJanuary 28, 2014Greet Hermans et al.normal rabbit serum and incubation with alkaline phosphatase-conjugated fast myosin antibodies (Sigma A4335, 1:500, 1 hour), and development (20 min) with the Vector Red Alkaline Phosphatase substrate kit (Vector Laboratories

(9) Interaction between Foxc1 and Fgf8 during Mammalian Jaw Patterning and in the Pathogenesis of SyngnathiaPLoS GeneticsDecember 19, 2013Kimberly E. Inman, Patricia Purcell, Tsutomu Kume, Paul A. Trainorserum (PBST-FBS) followed by incubation in alkaline phosphatase conjugated anti-Myosin (MY-32, Sigma, A4335), diluted to 10 µg/ml in PBST-FBS for 24 hours at 4°C. Heads were washed in PBST and alkaline phosphatase

(10) Hox11 genes are required for regional patterning and integration of muscle, tendon and boneDevelopment (Cambridge, England)November 1, 2013Swinehart IT, Schlientz AJ, Quintanilla CA, Mortlock DP, Wellik DM.(1:50, Developmental Studies Hybridoma Bank) and My32 (alkaline phosphatase conjugated, 1:800, Sigma, A4335; unconjugated, 1:200, Sigma, M4276)], lubricin (1:200, Thermo Scientific, PA3-118) and PECAM (1:10,

(11) Effects of Cell Grafting on Coronary Remodeling After Myocardial InfarctionJournal of the American Heart AssociationJune 21, 2013Jill J. Weyers, Stephen M. Schwartz, Elina Minami, Dara D. Carlson, Sarah K. Dupras, Kevin Weitz, Michael Simons, Timothy C. Cox, Charles E. Murry, William M. Mahoneymouse anti‐adult skeletal muscle myosin heavy chain II (clone MY32, alkaline phosphatase labeled, Sigma A4335, 1:400), rabbit anti‐mouse CD31 (Abcam ab28364, 1:100), rabbit anti‐eNOS (Abcam ab15280, undiluted),

(12) Slow-twitch fiber proportion in skeletal muscle correlates with insulin responsivenessThe Journal of clinical endocrinology and metabolismMay 1, 2013Charles A Stuart, Melanie P McCurry, Anna Marino, Mark A South, Mary E A Howell, Andrew S Layne, Michael W Ramsey, Michael H Stone

(13) A hydrogel derived from decellularized dermal extracellular matrixBiomaterialsOctober 1, 2012Matthew T. Wolf, Kerry A. Daly, Ellen P. Brennan-Pierce, Scott A. Johnson, Christopher A. Carruthers, Antonio D'Amore, Shailesh P. Nagarkar, Sachin S. Velankar, Stephen F. Badylakincubation in alkaline phosphatase conjugated mouse anti-fast myosin heavy chain (1:200, clone MY-32, A4335, Sigma) diluted in blocking solution for 1 hour. Color was developed by staining with alkaline phosphatase

(14) The effect of source animal age upon the in vivo remodeling characteristics of an extracellular matrix scaffoldBiomaterialsAugust 1, 2012Brian M Sicari, Scott A Johnson, Bernard F Siu, Peter M Crapo, Kerry A Daly, Hongbin Jiang, Christopher J Medberry, Stephen Tottey, Neill J Turner, Stephen F Badylak

(15) Biologic scaffold composed of skeletal muscle extracellular matrixBiomaterialsApril 1, 2012Matthew T. Wolf, Kerry A. Daly, Janet E. Reing, Stephen F. Badylakfollowed by incubation in alkaline phosphatase conjugated mouse anti-fast myosin heavy chain (1:200, A4335, Sigma) diluted in blocking solution for 1 h. After rinsing, color was developed by staining with red

(16) Nanoparticle based delivery of hypoxia-regulated VEGF transgene system combined with myoblast engraftment for myocardial repairBiomaterialsMarch 1, 2011Lei Ye, Wei Zhang, Li-Ping Su, Husnain K Haider, Kian-Keong Poh, Mary J Galupo, Geronica Songco, Ruo-Wen Ge, Huay-Cheem Tan, Eugene K W Sim

(17) Divergent transcriptional activities determine limb identityNature CommunicationsDecember 1, 2010Jean-François Ouimette, Marisol Lavertu Jolin, Aurore L'honoré, Anthony Gifuni, Jacques Drouinthe monoclonal alkaline phosphatase-conjugated anti-MHC diluted 1/100 in PBSMT (MY32 clone; cat. no. A4335, Sigma). After numerous washes in PBSMT first and then in NTMT (0.1 M NaCl, 0.1 M Tris (pH 9.5), 0.050

(18) Calpain-1 is required for hydrogen peroxide-induced myotube atrophyAmerican journal of physiology. Cell physiologyFebruary 1, 2009J M McClung, A R Judge, E E Talbert, S K Powers

(19) Angiomyogenesis using liposome based vascular endothelial growth factor-165 transfection with skeletal myoblast for cardiac repairBiomaterialsMay 1, 2008Lei Ye, Husnain Kh Haider, RuSan Tan, LiPing Su, Peter K Law, Wei Zhang, Eugene K W Sim

(20) Rosuvastatin: characterization of induced myopathy in the ratToxicologic pathologyFebruary 1, 2008F Russell Westwood, Robert C Scott, Alan M Marsden, Alison Bigley, Kevin Randall

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Monoclonal Anti-Myosin (Skeletal, Fast)−Alkaline Phosphatase antibody produced in mouse from MilliporeSigma

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Monoclonal Anti-Myosin (Skeletal, Fast)−Alkaline Phosphatase antibody produced in mouse from MilliporeSigma

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