Fig 1: Assessment of neuroepithelial marker, nestin in the second week of the differentiation protocol by: (A) immunofluorescence, and (B) flow cytometry. The results showed that the cells of all three experimental groups EEx, N, and NEx, were immunoreactive to nestin and the expression of this protein was higher in EEx and NEx groups. The cells in the E group showed no reactivity to nestin. DAPI was applied to counterstain the nuclei. NES, nestin; E (empty), EBs transfected with pCMV3-GFPSpark vector; EEx (empty/extract), EBs transfected with pCMV3-GFPSpark vector and treated by extract; N (Nurr), EBs transfected with pCMV3-NR4A2-GFPSpark vector; NEx (Nurr/extract), EBs transfected with pCMV3-NR4A2-GFPSpark vector and treated by extract.
Supplier Page from MilliporeSigma for Anti-Nestin antibody produced in rabbit