Fig 1: Effect of various stress in NFE and HPE on the GRP78 expression in 2-cell stage embryos in vitro. A GRP78 expression in 2-cell stage embryos exposed to pH stress. B Representative images of GRP78 expression pattern in 2-cell stage embryos exposed to pH stress studied by immunofluorescence (400 ×). C GRP78 expression in 2-cell stage embryos exposed to osmotic stress. D Representative images of GRP78 expression pattern in 2-cell stage embryos exposed to osmotic stress studied by immunofluorescence (400 ×). E GRP78 expression in 2-cell stage embryos exposed to oxidative stress. F Representative images of GRP78 expression pattern in 2-cell stage embryos exposed to oxidative stress studied by immunofluorescence (400 ×). Scale bar = 50 µM; **** p < 0.0001; a, p < 0.0001; b, p < 0.001. The number of embryos per group (n): pH stress, NFE pH 6.8 (n = 20), NFE pH 7.4 (n = 29), NFE pH 7.8 (n = 22), NFE pH 8.2 (n = 23), HPE pH 6.8 (n = 34), HPE pH 7.4 (n = 39), HPE pH 7.8 (n = 38), and HPE pH 8.2 (n = 33); osmotic stress, NFE isotonic (n = 56), NFE hypotonic (n = 53), NFE hypertonic (n = 52), HPE isotonic (n = 43), HPE hypotonic (n = 43), and HPE hypertonic (n = 40); and oxidative stress, NFE 0 µM H2O2 (n = 69), NFE 25 µM H2O2 (n = 68), HPE 0 µM H2O2 (n = 61), and HPE 25 µM H2O2 (n = 59)
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