Fig 1: NY-ESO-1 immunohistochemical staining pattern and intensity in liposarcomas and MPNSTPrimary myxoid/round cell liposarcoma, H&E (A) and diffuse (> 75% of cells staining) 3+ IHC positivity (B). Dedifferentiated liposarcoma, H&E (C) and 3+ IHC positivity in > 25% of cells (D). MPNST, H&E (E) and diffuse 3+ IHC positivity (F). Metastatic mxyoid/round cell liposarcoma, H&E (G) and diffuse 3+ IHC positivity (H). (200× magnification).
Fig 2: Decreased expression of HLA-A and antigen-presenting genes at progression.a Change in gene expression of CTAG1 (NY-ESO-1) and HLA-A between pre-infusion (n = 10 biologically independent samples) and at progression (n = 5 biologically independent samples). b Average expression and heatmap of antigen presentation genes at pre-infusion (n = 10 biologically independent samples) and progression (n = 5 biologically independent samples). The following genes had background expression across all samples: KLRC2, KIR3DL2, KIR3DL1, KIR2DS1, and KR2DL1. c Persistence of lete-cel in blood of patient 32. d Characterization of patient 32 biopsy taken 919 days post-infusion. RNAScope results show CD3 cells in blue and lete-cel in red (left) (representative region of tissue from an IHC run). PDL1 and LAG3 staining in brown by IHC (middle and right images). Tumor samples were primarily from lung metastases. Pre-infusion samples from seven archival screening samples (~1 year pre-infusion) and three fresh baseline samples (pre-lymphodepletion). At progression, samples consist of five samples. Box plots depict median as horizontal line within box, with box bounds as the first and third quartiles. Dots represent individual data points. Lower whisker is the minimum value of the data within 1.5 times the interquartile range below the 25th percentile. Upper whisker is the maximum value of the data within 1.5 times the interquartile range above the 75th percentile. Heatmaps show z-scores per gene. Nominal two-sided p-values obtained from linear mixed effects models (a, b). HLA human leukocyte antigen, IHC immunohistochemistry, NR non-responder, R responder.
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