Anti-Tubulin antibody produced in rabbit T3526 from MilliporeSigma

Product Specs
ItemAnti-Tubulin antibody produced in rabbit
CompanyMilliporeSigma
Price Supplier Page View Company Product Page
Catalog NumberT3526
Conjugate/Tagunconjugated
Reactivitymouse, chicken
TargetTuba1b, Tubb2b, Tubb2c, Tubb2a
Hostrabbit
Antibody Typeprimary antibodies
ApplicationsWB, IF-I
Clonalitypolyclonal
NCBI Full Gene Nametubulin, beta 2B class IIB, tubulin, beta 2A class IIA, tubulin, beta 4B class IVB, tubulin, alpha 1B
NCBI Gene Aliases4930542G03Rik, 2410129E14Rik, M(beta)2, Tuba2, Tubb2, Tubb2c, brdp, Tubb2c1

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MilliporeSigma

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Phone: (800)-645-5476
(800)-645-5439

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Website: www.SigmaAldrich.com/

(1) Somatic nuclear blebbing in Caenorhabditis elegans is not a feature of organismal aging but a potential indicator of germline proliferation in early adulthoodG3 Genes|Genomes|GeneticsApril 11, 2023Qiang Fan, Xue-Mei Li, Chao Zhai, Bin Li, Shang-Tong Li, Meng-Qiu Dongloading. Blots were incubated with anti-H3(1:5000, Cat#1791, Abcam) or anti-tubulin (1:5,000 dilution, Cat#T3526, Sigma-Aldrich). Quantification was carried out using ImageJ. Stress condition For heat stress, on

(2) Survival of compromised adult sensory neurons involves macrovesicular formationCell Death DiscoveryNovember 24, 2022Anand Krishnan, Aparna Areti, Prashanth Komirishetty, Ambika Chandrasekhar, Chu Cheng, Douglas W. Zochodne(1:600, Sigma Cat No. T8328) and rabbit monoclonal anti-βIII tubulin antibody (1:600, Sigma Cat No. T3526). Cells were incubated with the primary antibodies for 2 h and then washed with PBS. The corresponding

(3) Generation of stable microtubule superstructures by binding of peptide-fused tetrameric proteins to inside and outsideScience AdvancesSeptember 9, 2022Hiroshi Inaba, Yurina Sueki, Muneyoshi Ichikawa, Arif Md. Rashedul Kabir, Takashi Iwasaki, Hideki Shigematsu, Akira Kakugo, Kazuki Sada, Tomoya Tsukazaki, Kazunori Matsuuraincubated for 5 min, followed by a wash with 20 μl of BRB80. Then, 5 μl of anti-tubulin antibody (73 mg/ml; T3526, Sigma-Aldrich) was passed through the flow cell and incubated for 5 min. After washing with 20 μl of

(4) Insulin signaling regulates longevity through protein phosphorylation in Caenorhabditis elegansNature CommunicationsDecember 1, 2021Wen-Jun Li et al.probed with anti-GFP (1:3000 dilution, Cat#11814460001, Roche) or anti-tubulin (1:5000 dilution, Cat#T3526, Sigma-Aldrich) antibodies. Horseradish peroxidase-conjugated secondary antibodies (1:10,000 dilution,

(5) Oxidative stress transforms 3CLpro into an insoluble and more active form to promote SARS-CoV-2 replicationRedox biologyNovember 26, 2021Du L, Xie Y, Zheng K, Wang N, Gao M, Yu T, Cao L, Shao Q, Zou Y, Xia W, et al.mouse antibody (Proteintech, 60004-1-Ig), Anti-Tubulin antibody produced in rabbit (Sigma-Aldrich, T3526), Alpha tubulin monoclonal mouse antibody (Proteintech, 66031-1-Ig), goat anti-mouse antibody (LI-COR,

(6) Prenatal interleukin 6 elevation increases glutamatergic synapse density and disrupts hippocampal connectivity in offspringImmunityNovember 9, 2021Mirabella F, Desiato G, Mancinelli S, Fossati G, Rasile M, Morini R, Markicevic M, Grimm C, Amegandjin C, Termanini A, et al.,Cell Signaling Technology,Cat# 3230; RRID AB_2128522 Rabbit polyclonal anti-alphaTubulin,Sigma Aldrich,Cat# T3526; RRID AB_261659 "Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP",ThermoFisher,Cat# 31430; RRID AB_228307 "Goat

(7) RNA-binding protein Maca is crucial for gigantic male fertility factor gene expression, spermatogenesis, and male fertility, in DrosophilaPLOS GeneticsJune 28, 2021Li Zhu, Ryuya Fukunagaas below. Rabbit anti-Maca (1/10000, generated in this study), rabbit anti-Tubulin (1/1000, Sigma, T3526), mouse anti-alpha-Tubulin (1/1500, Sigma, PLOS Genetics | https://doi.org/10.1371/journal.pgen.1009655

(8) Increased hippocampal TrkA expression ameliorates cranial radiation‑induced neurogenesis impairment and cognitive deficit via PI3K/AKT signalingOncology ReportsSeptember 28, 2020Shengjun Ji, Haohao Wu, Xin Ding, Qingqing Chen, Xing Jin, Jinming Yu, Ming Yang(1:5,000; cat. no. ABS16), anti-β-actin (1:10,000; cat. no. AV40173) and anti-tubulin (1:10,000; cat. no. T3526) were purchased from Sigma-Aldrich, Merck KGaA. Goat anti-mouse HRP (1:10,000; code no. 115-035-166)

(9) Function of Cathepsin K in the Central Nervous System of Male Mice is Independent of Its Role in the Thyroid GlandCellular and Molecular NeurobiologyJuly 1, 2020Stephanie Dauth et al.stripped and re-probed for -tubulin, using a rabbit anti--tubulin primary antibody (Sigma-Aldrich Cat# T3526 RRID:AB_261659), and an HRP-conjugated goat anti-rabbit secondary antibody, as described above. Immunoreactions

(10) Effect of microtubule immobilization by glutaraldehyde on kinesin-driven cargo transportPolymer JournalJune 1, 2020Syeda Rubaiya Nasrin, Arif Md. Rashedul Kabir, Kazuki Sada, Akira Kakugomg/mL protein-A (P6031, Sigma-Aldrich, USA), followed by a 73 mg/mL anti-tubulin antibody solution (T3526, Sigma-Aldrich, USA), was applied to the flow cell. The flow cell was washed with solution A (~80 mM

(11) Dracorhodin perchlorate inhibits osteoclastogenesis through repressing RANKL‐stimulated NFATc1 activityJournal of Cellular and Molecular MedicineMarch 1, 2020Yuhao Liu et al.Antimouse IgG‐FITC antibody (Cat#F9137), anti‐vinculin antibody (Cat#V9264), anti‐tubulin antibody (Cat#T3526) and recombinant M‐CSF (Cat#M6518) were commercially available from Sigma‐Aldrich. Recombinant GST‐rRANKL

(12) Podocytes Produce and Secrete Functional Complement C3 and Complement Factor HFrontiers in immunologyJanuary 1, 2020Mühlig AK, Keir LS, Abt JC, Heidelbach HS, Horton R, Welsh GI, Meyer-Schwesinger C, Licht C, Coward RJ, Fester L, et al.number: ab24586), tubulin and laminin (both rabbit, 1:100, Sigma, St. Louis, MO, USA, catalog numbers: T3526, L9393) were used. Nuclei were stained with 4, 6-Diamidine-2-phenylindole dihydrochloride (DAPI) (1:10,000,

(13) Rspo2/Int7 regulates invasiveness and tumorigenic properties of mammary epithelial cellsJournal of Cellular PhysiologyDecember 25, 2019Malgorzata Klauzinska, Bolormaa Baljinnyam, Ahmed Raafat, Jaime Rodriguez-Canales, Luigi Strizzi, Yoshimi Endo Endo Greer, Jeffrey S. Rubin, Robert CallahanH-108) antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA). Rabbit anti-α-tubulin (1:5,000, #T3526) was from Sigma–Aldrich (St. Louis, MO). Alexa Fluor® 594 phalloidin (# A12381) was from Life Technologies

(14) Integrin-linked kinase controls retinal angiogenesis and is linked to Wnt signaling and exudative vitreoretinopathyNature communicationsNovember 20, 2019Park H, Yamamoto H, Mohn L, Ambühl L, Kanai K, Schmidt I, Kim KP, Fraccaroli A, Feil S, Junge HJ, et al.1: 5,000), anti-α-parvin (Abcam, ab11336, 1: 5000), anti-ILK (Abcam, ab49979, 1: 5000), anti-tubulin (Sigma, T3526, 1: 5000), anti-FLAG (Sigma, F7425, 1: 5000), anti-β-catenin (Cell signaling, #8814, 1:11,000), anti-AKT

(15) DEAD-box RNA helicase Belle posttranscriptionally promotes gene expression in an ATPase activity-dependent mannerRNA (New York, N.Y.)July 1, 2019Liao SE, Kandasamy SK, Zhu L, Fukunaga R.sources and dilutions of the primary antibodies were as below. Rabbit anti-Tubulin (1/1000, Sigma, T3526), mouse anti-α-tubulin (1/1500, Sigma, T9026), mouse anti-GFP (1/1000, Pierce, MA515256), mouse anti-HA

(16) EZH2-DNMT1-mediated epigenetic silencing of miR-142-3p promotes metastasis through targeting ZEB2 in nasopharyngeal carcinomaCell death and differentiationJune 1, 2019Li Y, He Q, Wen X, Hong X, Yang X, Tang X, Zhang P, Lei Y, Sun Y, Zhang J, et al.Cell Signaling Technology, 5246), GAPDH (1:5000; Abcam, ab128915), and a-tubulin (1:500; SigmaAldrich, Ronkonkoma, NY, USA, T3526). Horseradish peroxidase-conjugated antibody was used as the secondary antibody (anti-mouse or anti-rabbit;

(17) Pentraxin 3 regulates synaptic function by inducing AMPA receptor clustering via ECM remodeling and β1-integrinThe EMBO journalJanuary 3, 2019Fossati G, Pozzi D, Canzi A, Mirabella F, Valentino S, Morini R, Ghirardini E, Filipello F, Moretti M, Gotti C, et al.methanol, depending on the markers. The following antibodies were used: rabbit anti‐tubulin (1:100; T3526 Sigma‐Aldrich, Milan, Italy), guinea pig anti‐Bassoon (1:300; 141004, Synaptic Systems, Goettingen,

(18) LOTUS domain protein MARF1 binds CCR4-NOT deadenylase complex to post-transcriptionally regulate gene expression in oocytesNature communicationsOctober 2, 2018Zhu L, Kandasamy SK, Liao SE, Fukunaga R.as below. Rabbit anti-MARF1 (1/5000, generated in this study), rabbit anti-Tubulin (1/1000, Sigma, T3526), mouse anti-alpha-Tubulin (1/1500, Sigma, T9026), mouse anti-GFP (1/1000, Pierce, MA515256), mouse

(19) In vitro study on reversal of ovarian cancer cell resistance to cisplatin by naringin via the nuclear factor-κB signaling pathwayExperimental and therapeutic medicineMarch 1, 2018Zhu H, Gao J, Wang L, Qian KJ, Cai LP.h at room temperature. The membrane was then incubated with NF‑κ B, P‑gp and anti‑tubulin (cat. no. T3526; Sigma‑Aldrich; Merck KGaA, Darmstadt, Germany) primary antibodies overnight at 4˚C, and incubated with

(20) Tubulin or Not Tubulin: Heading Toward Total Protein Staining as Loading Control in Western BlotsPROTEOMICSOctober 1, 2017Christian P. MoritzAldrich 199.2 1000 5 200 1.00 Amido black A8181-1EA Sigma Aldrich 137 500 5 100 1.37 Anti-Tubulin antibody T3526-1ML Sigma Aldrich 882 1 0.025 40 22.05 Anti-Actin antibody A2066-.2ML Sigma Aldrich 390.5 0.2 0.05 4 97.63 HRP-coupled

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Anti-Tubulin antibody produced in rabbit from MilliporeSigma

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Anti-Tubulin antibody produced in rabbit from MilliporeSigma

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