Fig 2: Serial sections of primary tumours from humans with small-cell carcinoma in pulmonary bronchi. (A, B) representative serial sections from patient 1; (C, D) representative serial sections from patient 2. (A) and (C) show sections labelled with the anti-K8 antibody, TS1; (B) and (D) show sections labelled with an anti-SSTR2 antibody.

Fig 3: IHC analyses of NEC913 patient sample: (A) Primary NEC tumor at the ampulla of Vater. Scale bar represents 3 mm. (B) H&E staining of primary NEC tumor. (C–I) Staining for Ki67, CgA, SYP, SSTR2, ASCL1, p53, and Rb. Scale bar represents 200 µm.

Fig 4: Characterization of NEC913 and NEC1452 cells for NET markers: (A) NEC913 and NEC1452 cells incubated with antibodies against CgA (1/300), SSTR2 (1/300), and SYP (1/600) overnight and with secondary antibodies coupled to FITC (1/500) for 1 h at room temperature. Microscopy pictures are taken using 200 ms exposure time. Scale bar represents 100 μm. (B–H) Gene expression analyses of NET markers in NEC913 and NEC1452 cells compared to BON cells. Gene expression levels were normalized to the control gene GAPDH to determine the relative fold change. Statistical analyses of gene expression changes were performed using T-tests in Prism GraphPad. p < 0.05 was depicted with *. p < 0.01 was depicted with **.