Anti-α-Tubulin antibody, Mouse monoclonal T8203 from MilliporeSigma

Product Specs
ItemAnti-α-Tubulin antibody, Mouse monoclonal
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberT8203
Quantity100µL, 200µL, 25µL
Applicationsindirect ELISA, western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation (IP)
Conjugate/Tagunconjugated
Reactivityhuman, mouse, rat
TargetTUBA1A
CloneAA13
Hostmouse
ClonalityMonoclonal
Concentration~2.0 mg/mL
NCBI Full Gene Nametubulin, alpha 1A, tubulin alpha 1a
NCBI Gene AliasesTUBA3, Tuba1, LIS3, Tuba-1, B-ALPHA-1

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Website: www.SigmaAldrich.com/

(1) Attenuated Salmonella carrying siRNA-PD-L1 and radiation combinatorial therapy induces tumor regression on HCC through T cell-mediated immuno-enhancementCell death discoveryAugust 28, 2023Jia H, Wei P, Zhou S, Hu Y, Zhang C, Liang L, Li B, Gan Z, Xia Y, Jiang H, et al.CY5734; C-caspase3, 1:1000, CST, 9661S; C-caspase9, 1:2000, Abways, CY5682; Tubulin, 1:1000, Sigma, T8203) at 4 °C overnight. Secondary antibody (ZSGB-BIO, 1∶5000, ZB-2301 and ZB-2305) was incubated after finishing

(2) Filopodia-like protrusions of adjacent somatic cells shape the developmental potential of oocytesLife Science AllianceJune 1, 2023Flora Crozet, Gaëlle Letort, Rose Bulteau, Christelle Da Silva, Adrien Eichmuller, Anna Francesca Tortorelli, Joséphine Blévinal, Morgane Belle, Julien Dumont, Tristan Piolot, et al.Mounting Medium with DAPI (Ref. H-1200; Vector Laboratories). We used mouse anti-α-tubulin (1:200, Ref. T8203; Sigma-Aldrich) as primary antibodies and Cy3-conjugated donkey anti-mouse IgG (1:150, Ref. 715-165-151;

(3) Similar deamination activities but different phenotypic outcomes induced by APOBEC3 enzymes in breast epithelial cellsFrontiers in Genome EditingJune 1, 2023Milaid Granadillo Rodríguez, Lai Wong, Linda Chelicosame blot. The antibodies purchased from Sigma-Aldrich were anti-α-Tubulin antibody, mouse monoclonal (T8203/clone AA13, purified from hybridoma cell culture, Public ID AB_1841230, used at 1:1000); anti-α-Tubulin

(4) Effect of Postnatal Epigallocatechin-Gallate Treatment on Cardiac Function in Mice Prenatally Exposed to AlcoholAntioxidants (Basel)May 9, 2023Vicente Andreu-Fernández, Mariona Serra-Delgado, Laura Almeida-Toledano, Àgueda García-Meseguer, Melina Vieiros, Anna Ramos-Triguero, Concha Muñoz-Lozano, Elisabet Navarro-Tapia, Leopoldo Martínez, Óscar García-Algar, et al.Bcl-2-like protein 4 (Bax) (ref. 2771) were from Cell Signaling (Danvers, MA, USA); alpha-tubulin (ref. T8203, dilution 1:2000, 50 kDa) and anti-rabbit IgG secondary antibody (ref. A0545, dilution 1:2000) from

(5) Characterization of the functional and transcriptomic effects of pro-inflammatory cytokines on human EndoC-βH5 beta cellsFront Endocrinol (Lausanne)April 11, 2023Caroline Frørup, Rebekka Gerwig, Cecilie Amalie Søndergaard Svane, Joana Mendes Lopes de Melo, Kristine Henriksen, Tina Fløyel, Flemming Pociot, Simranjeet Kaur, Joachim Størlingtransducer and activator of transcription 1 (P-STAT1) (7649S, Cell Signaling, 1:1000), anti-Tubulin (T8203, Sigma-Aldrich, St. Louis, MO, USA, 1:2000), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (#9482,

(6) Mitochondrial pyruvate carrier inhibition initiates metabolic crosstalk to stimulate branched chain amino acid catabolismMolecular MetabolismApril 1, 2023Daniel Ferguson, Sophie J. Eichler, Nicole K.H. Yiew, Jerry R. Colca, Kevin Cho, Gary J. Patti, Trevor M. Shew, Andrew J. Lutkewitte, Sandip Mukherjee, Kyle S. McCommis, et al.(EMD Millipore AP1062), and PDH (CST 3205); BDK (sc-374,425) and PPM1K (sc-514,925); and α-Tubulin (Sigma T8203). Images were obtained using the Licor system with Image Studio Lite software. 2.8 mTOR phospho antibody array

(7) The importance of nuclear RAGE-Mcm2 axis in diabetes or cancer-associated replication stressNucleic acids researchMarch 21, 2023Han Z, Andrš M, Madhavan BK, Kaymak S, Sulaj A, Kender Z, Kopf S, Kihm L, Pepperkok R, Janscak P, et al.,Cell Signaling Technology,Cat#13110S RPA2,ABGENT,Cat#AP9115a Tubulin,Thermo Fisher Scientific,Cat#14–4510-80 Acetylated-Tubulin,Sigma-Aldrich,Cat#T8203 Ki67,Thermo Fisher Scientific,Cat#14-5698-82 anti-BrdU antibody,Abcam,Cat#ab6326 anti-BrdU antibody,BD Biosciences

(8) CLIC and membrane wound repair pathways enable pandemic norovirus entry and infectionNature communicationsFebruary 28, 2023Ayyar BV, Ettayebi K, Salmen W, Karandikar UC, Neill FH, Tenge VR, Crawford SE, Bieberich E, Prasad BVV, Atmar RL, et al.was stained using Alexa-647 Phalloidin and tubulin was stained using mouse anti-tubulin antibody (#T8203, Sigma) followed by using goat anti-mouse 594 antibody(#610-742-124, Rockland) as a secondary antibody.

(9) HNRNPA2B1-Mediated MicroRNA-92a Upregulation and Section Acts as a Promising Noninvasive Diagnostic Biomarker in Colorectal CancerCancersFebruary 21, 2023Li Y, Li K, Lou X, Wu Y, Seery S, Xu D, Pei Y, Qian B, Wu Y, Liang S, et al.against HNRNPA2B1 (1:200, sc-53531, Santa Cruz Biotechnology, Dallas, TX, USA) and alpha-Tublin (1:2000, t8203, Sigma, St. Louis, MO, USA). Further, mouse monoclonal antibodies were blotted at room temperature for

(10) Melatonin protects against cadmium-induced oxidative stress via mitochondrial STAT3 signaling in human prostate stromal cellsCommunications BiologyFebruary 8, 2023Moonjung Hyun, Hyejin Kim, Jehein Kim, Juhong Lee, Ho Jeong Lee, Laxmi Rathor, Jeremy Meier, Andrew Larner, Seon Min Lee, Yeongyu Moon, et al.#ab14734, 1 mg/ml, diluted 1:1,000), Grim-19 (Abcam, #ab110240, 1 mg/ml, diluted 1:1,000), and tubulin (#T8203; Sigma-Aldrich, 2 mg/ml, 1:5,000). After incubation with secondary antibodies (anti-mouse NA931V or

(11) Drosophila Ectoderm-expressed 4 modulates JAK/STAT pathway and protects flies against Drosophila C virus infectionFrontiers in ImmunologyFebruary 3, 2023Zongliang Huang, Wei Wang, Pengpeng Xu, Shangyu Gong, Yingshan Hu, Yan Liu, Fang Su, Khalid Mahmood Anjum, Wu-Min Deng, Suping Yang, et al.Proteintech 66007-1-Ig); mouse anti-HA (1:8000, Milipore 05-904); mouse anti-α-Tubulin (1:20,000 Sigma T8203); goat anti-Stat (1:5000, Santa Cruz Biotechnology dN-17); mouse anti-FLAG (1:8000, Sigma F3165); rabbit

(12) Carbonic anhydrases in development: morphological observations and gene expression profiling in sea urchin embryos exposed to acetazolamideOpen BiologyJanuary 4, 2023Francesca Zito, Rosa Bonaventura, Caterina Costa, Roberta Russoc-jun (1 : 500; Aviva Systems Biology, Cat# ARP30926_P050), α-tubulin (1 : 500; Sigma-Aldrich Cat# T8203, RRID:AB_1841230). The secondary antibodies IRDye 680RD goat anti-rabbit (1 : 5000, LI-COR Biosciences

(13) Mitochondrial decline in the ageing old world primate retina: Little evidence for difference between the centre and peripheryPloS oneJanuary 1, 2023Kam JH, Shinhmar H, Powner MB, Hayes MJ, Aboelnour A, Jeffery G.,Santa Cruz Biotechnology Inc. Mouse monoclonal to COXIV (Complex IV),Ab33985,1 μg/ml,10 μg/ml,Abcam Mouse monoclonal to α-tubulin,T8203,0.4 μg/ml,,Sigma-Aldrich Rabbit polyclonal to GAPDH,ABS16,0.245 μg/ml,,Milipore

(14) Protein misfolding and clearance in the pathogenesis of a new infantile onset ataxia caused by mutations in PRDX3Human molecular geneticsNovember 10, 2022Martínez-Rubio D, Rodríguez-Prieto Á, Sancho P, Navarro-González C, Gorría-Redondo N, Miquel-Leal J, Marco-Marín C, Jenkins A, Soriano-Navarro M, Hernández A, et al.dilutions used in WB were as follows: anti-FLAG M2® (F3165, Sigma-Aldrich): 1/3000; anti-α-tubulin (T8203, Santa Cruz Biotechnology, Dallas, TX, USA): 1/3000; anti-PRDX3 (PA1835, Boster Biological Technology,

(15) Sleep and wake cycles dynamically modulate hippocampal inhibitory synaptic plasticityPLoS biologyNovember 1, 2022Wu K, Han W, Lu W.Synaptic Systems), Mouse monoclonal anti-Gephyrin (Cat# 147021, Synaptic Systems), Mouse monoclonal α-tubulin (Cat# T8203, Sigma-Aldrich), Mouse monoclonal Anti-Glutamate Receptor NMDAR1 (GluN1) (Cat# MAB363, Sigma-Aldrich),

(16) A small molecule inhibitor of the UBE2F-CRL5 axis induces apoptosis and radiosensitization in lung cancerSignal transduction and targeted therapyOctober 17, 2022Xu T, Ma Q, Li Y, Yu Q, Pan P, Zheng Y, Li Z, Xiong X, Hou T, Yu B, et al.CST), γH2AX (05-636, EMD Millipore), DAPI (D1306, Invitrogen), β-Actin (M1210-2, HuaBio) and α-Tubulin (T8203, Sigma-Aldrich). Docking simulations All docking simulations were carried out by the Glide module

(17) Mutation in senataxin alters the mechanism of R-loop resolution in amyotrophic lateral sclerosis 4Brain : a journal of neurologySeptember 14, 2022Kannan A, Cuartas J, Gangwani P, Branzei D, Gangwani L.immunoblot analysis, SMN (610647, clone 8) from BD Biosciences, ZPR1 (Clone LG-C61),32,35 α-tubulin (#T8203) from Sigma-Aldrich, γH2AX (phospho Ser139) (ab26350), p-DNA-PKcs (Ser2056) (ab18192) and SETX (ab220827),

(18) Unfolding and identification of membrane proteins in situeLifeSeptember 12, 2022Nicola Galvanetto, Zhongjie Ye, Arin Marchesi, Simone Mortal, Sourav Maity, Alessandro Laio, Vincent Aldo Torremilk. The membranes were incubated with the corresponding primary antibodies 1:5000 for α-Tubulin (Cat# T8203, Sigma); 1:500 for TRPC1 (Cat# ACC-101, Alomone Labs)/5 (Cat# ACC-020, Alomone Labs)/6 (Cat#ACC-120,

(19) Development of a novel peptide to prevent entry of SARS-CoV-2 into lung and olfactory bulb cells of hACE2 expressing miceMolecular brainAugust 9, 2022Su P, Zhai D, Wong AHC, Liu F.GTX632604), anti-hACE2 (R&D Systems, goat, Cat No. AF933), anti-α-Tubulin (Sigma-Aldrich, mouse, Cat No. T8203), normal mouse IgG (Santa Cruz Biotechnology, Cat No. sc-2025), HRP-conjugated anti-goat secondary antibody

(20) Specific Targeting of Antiapoptotic Bcl-2 Proteins as a Radiosensitizing Approach in Solid TumorsInternational Journal of Molecular SciencesJuly 16, 2022Benjamin Sobol et al.Cell Signaling Technology, Danvers, MA, USA), pH2AX (# 9718, Cell Signaling Technology, Danvers, MA, USA), Tubulin (# T8203, Sigma–Aldrich, St. Luis, MO, USA), and Actin (# sc-1616, Santa Cruz Biotechnology, Heidelberg, Germany).

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Anti-α-Tubulin antibody, Mouse monoclonal from MilliporeSigma

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Anti-α-Tubulin antibody, Mouse monoclonal from MilliporeSigma

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