Fig 1: Western blotting analysis of URAT1, GLUT9, OAT4 and ABCG2 in the kidney cortex tissues. Data are presented as mean ± SEM, n = 5/group. #p< 0.05 vs. 200 mg/kg inosine-treated hyperuricemic monkeys. *p< 0.05 vs. control monkeys.
Fig 2: Reverse transcription-qPCR analysis of URAT1, GLUT9, OAT4 and ABCG2 in the kidney cortex tissues. Data are presented as mean ± SEM, n = 5/group.
Fig 3: Effect of ethanol (80%) extract of A. paniculata leaves, andrographolide and allopurinol on protein expressions of renal URAT1 (A), GLUT9 (B) and OAT1 (C) in hyperuricemic-induced rats obtained from Western blot analysis (D). Data are presented as mean ± SEM (n = 6). Data were analyzed using one-way ANOVA followed by post hoc Tukey. aSignificantly different compared to normal group (p < 0.05). bSignificantly different compared to hyperuricemic group (p < 0.05).
Fig 4: (A1–C1): ABCC4 and ABCG2 protein expression, normalized against GAPDH, in rat’s kidney. Data are means ± SD (n = 8). * Significantly different from CON (p < 0.05), ** Significantly different from CON (p < 0.01). (A2–C2): URAT1 and GLUT9 protein expression, normalized against GAPDH, in NRK-52E cells 24 h after exposure to 10% serum without exercise (CON), serum with exercise (EXE) or serum with exercise supplemented with ML385. Data are means ± SD (n = 6). * Significantly different from CON (p < 0.05), # Significantly different from EXE (p < 0.05).
Fig 5: Conditional deletion of Glut1 in NP compartment of adult mice.(A) Schematic showing K19CreERT-mediated deletion of Glut1/Slc2a1 exons 3–8 to generate NP-specific Glut1 mutant. (B) Quantification of Glut1 in cKOK19 mice (n = 7 mice/genotype; 20 discs/animal). (C) Representative IHC images and quantification of GLUT1 in 9-month-old WT and Glut1cKOK19 (scale bar = 200 µm and 50 µm) (n = 8 WT, 7 cKO mice; 6 lumbar and 3 caudal discs/animal). White dotted lines demarcate disc compartments. (D and E) Western blot and quantification of GLUT1 and GLUT9 levels in NP and AF tissues of WT and Glut1cKOK19 mice (n = 5 mice/genotype; 20 discs/animal); blots were first probed for GLUT1, stripped, and reprobed for GLUT9. Western blot and quantification of GLUT3 in NP and AF (n = 5 mice/genotype; 20 discs/animal). (F) qRT-PCR of Glut3, Glut9, and Sglt1 in WT and Glut1cKOK19 mice (n = 7 mice/genotype; 20 discs/animal). Quantitative measurements represent mean ± SEM; the significance of differences was determined using Mann-Whitney U test.
Supplier Page from Abcam for Anti-GLUT9 antibody