Fig 1: (A) Western blot analysis of S1PR1 expression in SK-HEP-1, MCF-7 tumor tissues and muscle. (B–I) The immunohistochemical staining results of various organs and tissues (under 100× and 200× magnification).
Fig 2: (A) Western blot analysis of S1PR1 expression in SK-HEP-1 cells and MCF-7 cells; (B) Stability of 99mTc-HYNIC-S1PR1mAb in vitro at 3h, 6h, 9h, 12h in FBS; (C) In vitro cellular uptake. The total bindings and blocking results of 99mTc-HYNIC-S1PR1mAb in SK-HEP-1 cells (experiment group) and MCF-7 cells (control group) were included in panel C, as well as the specific binding in SK-HEP-1 cells. There was no significant difference between the blocking and non-blocking in MCF-7 cells. *, **, and ns indicate P < 0.05 and P > 0.05 (n = 4), respectively.
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