Fig 1: Iron and ferroptosis-related proteins are upregulated in BPD. A: representative brightfield Prussian blue staining for iron in whole tissue sections from control (left), hBPD (middle), and eBPD (right). B: quantification of Prussian blue+ cells show increased iron deposition in eBPD vs. other groups. C: Western blots, control n = 6, hBPD n = 3, eBPD n = 3, (dotted line in FTL blot indicates where empty lane was removed from the image). See Supplemental Fig. S3, A–F, for labeled unedited blots. D: quantification of Western blots for FTH1 (top), FTL (middle), and GAPDH loading control (bottom) for each sample in the experimental groups. E: mass spectrometry intensities for iron and ferroptosis-related proteins in the data set. Stars indicate t test P values (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001) performed between groups indicated by brackets. BPD, bronchopulmonary dysplasia; eBPD, established bronchopulmonary dysplasia; hBPD, healed bronchopulmonary dysplasia.
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