Fig 1: The Hippo signaling pathway in BMSCs was mediated by MPS. The protein expression levels of MST1, p-MST1, Yap, p-Yap, LATS1/2, p-LATS1/2, TAZ, and p-TAZ were detected by western blot. *p < 0.05, **p < 0.01, ***p < 0.001. The experiment was repeated three times.
Fig 2: Downregulated MST1 alleviated the effects of osteogenesis and Hippo signaling and enhanced adipogenesis in BMSCs. (a) The calcium nodule form in BMSCs was detected by Alizarin Red S staining analysis. (b) The mRNA expression levels of OCN, OPN, and Runx2 in BMSCs treated with MPS were evaluated by qRT-PCR. (c) The ALP activity in MPS conditions was detected by ALP activity assay. (d) Oil droplets in BMSCs were observed using Oil Red O staining (×100). (e) The mRNA expression of PPARγ was evaluated by qRT-PCR. (b) The content of TG was measured in BMSCs. (f) The content of TG was measured in BMSCs. (g) The protein expression levels of MST1, p-MST1, Yap, p-Yap, LATS1/2, p-LATS1/2, TAZ, and p-TAZ were detected by western blot. *p < 0.05, **p < 0.01, ***p < 0.001. The experiment was repeated three times.
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