Fig 1: Consequences of myeloid cell AMPKa2 deletion on the S-adenosyl methionine metabolism. (A) Results of the proteomics experiments using monocytes from wild-type (WT) and AMPKa2?MC (?MC) mice exposed to hypoxia (1% O2; 16 h); n = 6 animals per group. (B) MAT2B mRNA expression in macrophages from wild-type (WT) and AMPKa2?MC (?MC) mice; n = 16 animals per group (each sample in duplicate). (C) MAT2B protein expression in macrophages from wild-type (WT) and AMPKa2?MC (?MC) mice; n = 13–16 animals per group. (D) SAM/SAH ratio in macrophages from wild-type (WT) and AMPKa2?MC (?MC) mice; n = 13 animals per group. (E) Methyl cytosine levels in total genomic DNA from macrophages from wild-type (WT) and AMPKa2?MC (?MC) mice; n = 6 animals per group. (F) DNMT1 RNA expression, n = 11 animals per group (each sample in duplicate) and (G) protein expression, n = 10 animals per group. (Students t-test) * p < 0.05, ** p < 0.01, *** p < 0.001.