Fig 1: Olaparib potentiates ?H2A.X and RAD51 recruitment for strand break induced by P8-D6 in OvCar8. (A) Immunofluorescence images of OvCar8 2D monolayer: DAPI-stained nuclei (blue), ?H2A.X (green) and RAD51 (red) after treatment with olaparib and/or P8-D6; scale bars, 50 µm. (B) The intensity of the ?H2A.X and RAD51 signal in relation to DAPI in OvCar8 2D monolayer after treatment. (C) Confocal fluorescence z-stack images of OvCar8 spheroids were obtained with immunostaining against ?H2A.X, RAD51 and Hoechst 33342. Spheroids were treated with olaparib and/or P8-D6, permeabilized with glycerol, followed by antibody staining; scale bars, 100 µm. (D) ?H2A.X and RAD51 fluorescence intensities of different treatments were compared between different treatments.