Fig 1: WTC dust exposure promotes immune cell infiltration in the lungs and prostates of normal and GEM models of prostate cancer. A, Low magnification H&E images harvested from C57BL/6 mice 3 months after exposure to WTC dust or control PBS control. Significant increases in the presence of lymphoid aggregates are present in the lungs of dust-exposed mice as compared with controls (left bar = 500 μm). B, Immune aggregates counted in tissue sections of lungs harvested from WTC (n = 5) and PBS (n = 5) exposed mice. Aggregates exceeding 20 μm were counted. C, Lungs from mice with WTC and PBS treatments showing the accumulation of lymphoid aggregates composed of high numbers of CD19+ B cells, CD3+ T cell, and NK1.1+ NK cells (black bar = 50 μm, white bar = 100 μm). D, Evaluation of immune cell infiltration by flow cytometry in normal and Pb-Cre+;PtenL/L GEM prostates shown for mice 12 weeks after WTC or PBS exposure (n = 7–10 per cohort). E, Evaluation of immune cell infiltration using Qupath analysis of scanned H&E-stained tissues for WTC- and PBS-treated samples (left bar = 500 μm, right bar = 100 μm). F, Measured values for immune cell occupancy Wt and Pten-mutant mice exposed at different timepoints shown as a percentage of the total tissue area (bottom).