Fig 1: MCA reduced mitochondrial fragmentation. (a) Representative images of mitochondrial morphology after cocultured. The mitochondria can be classified into small globular, linear tubular, branched tubular, twisted tubular, and looped types, using MicroP software. The two major types of mitochondria, the globular and tubular, were enumerated and the results were shown in (b). In coculture containing MCA-treated hMSCs, the proportion of globular mitochondria decreased, while the proportion of the healthier tubular mitochondria increased significantly. (c) The length of the mitochondria in the coculture containing MCA-treated hMSCs was longer than that in the control hMSCs coculture. The width of the mitochondria from both coculture did not differ. (d) Western blot analysis of Drp1 S616 and Mfn2. The fold ratios of lanes 2 and 3 were obtained after comparing to lane 1, while that of lane 5 were obtained after comparing to lane 4. *p < 0.05, **p < 0.01, ***p < 0.001, n.s., not significant. Scale bar: 20 µm.