Anti-Grp75/MOT antibody [JG1] ab2799 from Abcam

Product Specs
ItemAnti-Grp75/MOT antibody [JG1]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab2799
Antibody TypePrimary
HostMouse, Nude mouse
CloneJG1
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
IsotypeIgG3
ClonalityMonoclonal
Quantity100 UL
Conjugate/TagUnconjugated
Concentration2.9 mg/ml
Gene NameHSPA9
ReactivityHuman, Mouse
TargetGrp75 / MOT

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) NPC1-dependent alterations in KV2.1–CaV1.2 nanodomains drive neuronal death in models of Niemann-Pick Type C diseaseNature CommunicationsJuly 28, 2023Maria Casas, Karl D. Murray, Keiko Hino, Nicholas C. Vierra, Sergi Simó, James S. Trimmer, Rose E. Dixon, Eamonn J. DicksonBLOCK and 0.5% Triton X‐100 in PBS with the following primary antibodies: GRP75 1:100 (Abcam, Cat #ab2799), KV2.1 10 µg/mL (NeuroMab, K89/34), CaV1.2 1:333 (Alomone, Cat #acc-003), KV2.1(pS603) 1:5 (L61/14.2),

(2) Aerobic Exercise Delays Alzheimer’s Disease by Regulating Mitochondrial Proteostasis in the Cerebral Cortex and HippocampusLife (Basel)May 17, 2023Kaiyin Cui, Chaoyang Li, Guoliang Fangnonfat milk powder) overnight at 4 °C. The blots were probed using the following antibodies: HSPA9 (ab2799, 1:1000, Abcam, Cambridge, UK), HSP60 (ab46798, 1:5000, Abcam, Cambridge, UK), HSP10 (ab109624, 1:1000,

(3) Fumarate induces vesicular release of mtDNA to drive innate immunityNatureMarch 1, 2023Zecchini V, Paupe V, Herranz-Montoya I, Janssen J, Wortel IMN, Morris JL, Ferguson A, Chowdury SR, Segarra-Mondejar M, Costa ASH, et al.polyclonal anti-FH/fumarase (ab113963) and anti-β-actin (AC-15) (ab6276), rabbit polyclonal anti-GRP75 (ab2799), anti-MFN1 (ab126575), anti-phospho-IRF3S386 (ab76493) and mouse monoclonal anti-VDAC1 (ab14734) antibodies

(4) GRP75 as a functional element of cholix transcytosisTissue BarriersJanuary 2, 2023Keyi Liu, Tom Hunter, Alistair Taverner, Kevin Yin, Julia MacKay, Kate Colebrook, Morgan Correia, Amandine Rapp, Randall J. Mrsnyand ImageJ. Antibodies and recombinant proteins Primary antibodies: Mouse anti-GRP75 mAb (Abcam, ab2799); mouse anti-hsp60 mAb (Abcam, ab110312); mouse anti-GRP78 mAb (Abcam, ab25192); mouse anti-Hsp90 mAb

(5) AMPK-dependent phosphorylation of MTFR1L regulates mitochondrial morphologyScience advancesNovember 11, 2022Tilokani L, Russell FM, Hamilton S, Virga DM, Segawa M, Paupe V, Gruszczyk AV, Protasoni M, Tabara LC, Johnson M, et al.anti-SDHA (ab14715), anti-ATP5a (ab14748), anti-MTCO2 (ab110258), anti-Mic60 (ab110329), and anti-GRP75 (ab2799) antibodies were obtained from Abcam. Rabbit polyclonal anti-Mid49 (16413-A-AP), anti-Mid51 (20164-A-AP),

(6) PolyGA targets the ER stress-adaptive response by impairing GRP75 function at the MAM in C9ORF72-ALS/FTDActa NeuropathologicaNovember 1, 2022Federica Pilotto, Alexander Schmitz, Niran Maharjan, Rim Diab, Adolfo Odriozola, Priyanka Tripathi, Alfred Yamoah, Olivier Scheidegger, Angelina Oestmann, Cassandra N. Dennys, et al.buffered saline PBS. iMNs were incubated with the following primary antibody: mouse anti-GRP75 (Abcam, ab2799, 1:200), rabbit anti-GRP75 (1:200, Abcam, ab53098), rabbit anti-BiP (Abcam, ab21685, 1:500), goat anti-ChAT

(7) Protein import motor complex reacts to mitochondrial misfolding by reducing protein import and activating mitophagyNature communicationsSeptember 2, 2022Michaelis JB, Brunstein ME, Bozkurt S, Alves L, Wegner M, Kaulich M, Pohl C, Münch C.12720-1-AP, dilution 1:1000) anti-HSP60 (Abcam, ab4679, dilution 1:10,000) anti-HSPA9 (Abcam, JG1 clone, ab2799, dilution 1:2000) anti-LONP1 (Proteintech,15440-1-AP, dilution 1:1000) anti-PAM16 (Proteintech, 15321-1-AP,

(8) Notch1 Modulation of Cellular Calcium Regulates Mitochondrial Metabolism and Anti-Apoptotic Activity in T-Regulatory CellsFrontiers in ImmunologyFebruary 10, 2022Neetu Saini, Sowmya Lakshminarayanan, Priyanka Kundu, Apurva Sarin14997S), Vps34 (D9A5, 4263) were from Cell Signaling Technology (MA, USA). Antibodies to Grp75 (JG1, Ab2799), VDAC1 (20B12AF2, Ab14734), pPDH (EPR12200, Ab177461) and Notch1 (mN1A, 128076) were from Abcam (Cambridge,

(9) Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machineryMolecular cellJanuary 20, 2022Schäfer JA, Bozkurt S, Michaelis JB, Klann K, Münch C.Primary antibodies; HSPD1 (Abcam ab46798, 1/1000), ATP5A1 (Proteintech 14676-1-AP, 1/1000), HSPA9 (Abcam ab2799, 1/1000), SDHA (Proteintech 14865-1-AP, 1/1000), COX5B (Proteintech 11418-2-AP, 1/1000), COXIVL1 (Proteintech

(10) An anaplerotic approach to correct the mitochondrial dysfunction in ataxia-telangiectasia (A-T)Molecular metabolismDecember 1, 2021Yeo AJ, Subramanian GN, Chong KL, Gatei M, Parton RG, Coman D, Lavin MF.(GTX70107; Genetex, CA, USA), anti-ATM phospho-Ser1981 (ab81292; Abcam, Cambridge, U.K), anti-Grp75 (ab2799; Abcam, Cambridge, U.K), anti-VDAC1 (ab15895; Abcam, Cambridge, U.K), anti-IP3R1 (ab5804; Abcam, Cambridge,

(11) Quantifying inter-organelle membrane contact sites using proximity ligation assay in fixed optic nerve sectionsExperimental Eye ResearchDecember 1, 2021Jared Ching, Andrew Osborne, Richard Eva, Julien Prudent, Patrick Yu-Wai-Man(Abcam, ab137029, 1:300) binds Rab7 GTPase located on late endosomes, and mouse anti-Grp75 (Abcam, ab2799, 1:300) binds the chaperone glucose-regulated protein-75 located at both the mitochondrial and ER when

(12) Drp1-dependent peptide reverse mitochondrial fragmentation, a homeostatic response in Friedreich ataxiaPharmacology research & perspectivesMay 1, 2021Johnson J, Mercado-Ayón E, Clark E, Lynch D, Lin H.(Cell Signaling Technology, 3455S, 1:250 for ICC, 1:1000 for WB), α‐FXN (Abcam, ab110328, 1:1000 for WB), α‐GRP75 (Abcam, ab2799, 1:250 for ICC). 2.8 Western blot Twenty micrograms of cell lysate were loaded into each well of a

(13) LOXL1‑AS1 promotes thymoma and thymic carcinoma progression by regulating miR‑525‑5p‑HSPA9Oncology ReportsApril 27, 2021Jin Wang, Haihua Huang, Xiaomiao Zhang, Haitao Mamin. The PVDF membrane was blocked in 5% nonfat milk for 1 h at room temperature. The HSAP9 antibody (ab2799; Abcam; 74 kDa) and GAPDH antibody (ab8245; Abcam) were diluted at 1:1,000 with 5% BSA and added to

(14) HN1L promotes migration and invasion of breast cancer by up‐regulating the expression of HMGB1Journal of Cellular and Molecular MedicineJanuary 1, 2021Dechuang Jiao, Jingyang Zhang, Ping Chen, Xuhui Guo, Jianghua Qiao, Jiujun Zhu, Lina Wang, Zhenduo Lu, Zhenzhen Liu(Cell Signaling Technology, #3932s), β‐catenin (Cell Signaling Technology, #9562), Twist1 (Abcam, ab50581), HSPA9 (Abcam, ab2799) and GAPDH (Santa Cruz, sc‐32233), followed by incubating with secondary antibodies labelled with horseradish

(15) Combinatory Treatment of Canavanine and Arginine Deprivation Efficiently Targets Human Glioblastoma Cells via Pleiotropic MechanismsCellsSeptember 30, 2020Olena Karatsai, Pavel Shliaha, Ole N. Jensen, Oleh Stasyk, Maria Jolanta Rędowiczβ-tubulin (V4505 and T0198, respectively; Sigma-Aldrich), GRP78 BiP (ab21685), GFAP (ab7260), GRP75/MOT (ab2799), lamin B1 (ab16048) and lamin B2 (ab151735) (Abcam, Cambridge, UK), and GAPDH (Millipore MAB374, St.

(16) DRP-1 functions independently of mitochondrial structural perturbations to facilitate BH3 mimetic-mediated apoptosisCell Death DiscoveryDecember 1, 2019Mateus Milani, Alison J. Beckett, Aoula Al-Zebeeby, Xu Luo, Ian A. Prior, Gerald M. Cohen, Shankar VaradarajanKong) and A-1331852 from AbbVie Inc. (North Chicago, IL, USA) were used. Antibodies against HSP70 (cat#ab2799) from Abcam (Cambridge, UK), OPA1 (cat#612607), cytochrome c (cat#556432) and DRP-1 (cat#611113) from

(17) Pptc7 is an essential phosphatase for promoting mammalian mitochondrial metabolism and biogenesisNature CommunicationsDecember 1, 2019Natalie M. Niemi et al.(Abcam #ab18988, dilution 1:2000), anti-OxPhos (Abcam #ab110412, dilution 1:2000), anti-GRP75 (Abcam #ab2799, dilution (1:500), anti-PMPCB (dilution 1:250), anti-Tom70p (dilution 1:500) and anti-Tom20p (dilution

(18) Proteasome dysfunction induces excessive proteome instability and loss of mitostasis that can be mitigated by enhancing mitochondrial fusion or autophagyAutophagyOctober 1, 2019Tsakiri EN, Gumeni S, Vougas K, Pendin D, Papassideri I, Daga A, Gorgoulis V, Juhász G, Scorrano L, Trougakos IP.The antibodies against NDUFS3 (ab14711), ATP5PF/complex V subunit-ATP5F1A (ab14748) and HSPA9/Grp75 (ab2799) were from Abcam and the antibody against PSMD11 (Rpn6, NBP1-46191) was from Novus. Statistical analyses Experiments

(19) PCDHGB7 Increases Chemosensitivity to Carboplatin by Inhibiting HSPA9 via Inducing Apoptosis in Breast CancerDisease MarkersJuly 8, 2019Siqi Hou, Ming Shan, Chunyang Gao, Xinxin Feng, Yongheng Yang, Ruo Zhang, Yan He, Guoqiang Zhang, Lei ZhangPVDF membranes (Bio-Rad, USA). The membranes were incubated with mouse monoclonal antihuman HSPA9 (ab2799, Abcam, USA, 1 : 1000), rabbit polyclonal anti-active caspase-3 (ab2302, Abcam, USA), and rabbit monoclonal

(20) HIF-1α protects against oxidative stress by directly targeting mitochondriaRedox biologyJuly 1, 2019Li HS, Zhou YN, Li L, Li SF, Long D, Chen XL, Zhang JB, Feng L, Li YP.immunofluorescence of tissues, Bcl-XL (ab32370, Abcam, Cambridge, UK), cytochrome c (ab76237, Abcam), Grp75 (ab2799, Abcam), translocase of outer mitochondrial membrane 40 (TOMM40; ab185543, Abcam), histone deacetylase

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Anti-Grp75/MOT antibody [JG1] from Abcam

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Anti-Grp75/MOT antibody [JG1] from Abcam

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