Fig 1: The specificity of the CHOP antibodies. (a) INS 832/13 cells expressing GFP (G), r-IAPP (R), or h-IAPP (H) were treated with 0.5 μg/ml tunicamycin and fractionated into cytoplasmic and nuclear fractions. (b) INS cells were cultured in the presence or absence of 1 or 5 μg/ml tunicamycin (TM) for 6 h and fractionated. Lysates were analyzed by immunoblotting using different CHOP antibodies. Positive staining was also obtained using CHOP antibody 9C8 (cat#ab11419, Abcam). Non-specific signal in our testing conditions in INS cells was obtained using CHOP antibodies cat#G6916 (lot#57K4864, Sigma) and cat#sc-793R-20 (lot# F0105, Santa Cruz). The filters were also probed for PARP and GAPDH to show successful fractionation to nuclear and cytoplasmic fractions, respectively. (c) Human IAPP expressing INS cells (upper panel) and pancreatic sections of human IAPP transgenic mouse (lower panel) were stained with different CHOP antibodies. Positive staining was also obtained using CHOP antibody 9C8 (cat#ab11419, Abcam)
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