Fig 1: Effects of MitoQ on compression-induced alterations in mitochondrial dynamics in human NP cells. (A-B) Human NP cells were pre-treated with MitoQ (500 nmol/L) for 2 h and then exposed to compression for 36 h. The colocalization of Drp1 and Tom20 was examined by confocal microscopy. Scale bar: 10 µm. (C-D) The protein levels of mitochondrial Drp1 (mito-Drp1) and cytoplasmic Drp1 (cyto-Drp1) in the human NP cells were measured by Western blotting. (D-F) The protein levels of total Drp1, Mff, Fis1, Mid49, Mid51, Mfn1, Mfn2 and Opa1 in the human NP cells were measured by Western blotting. Data are represented as the mean ± SD. ***P < .001, **P < .01, *P < .05, n = 3