Fig 1: S. aureus induces loss of cholesterol in macrophages in the infected-bone of mice. (A) Heatmap showing relative expression of CMGs in S. aureus infected femur on day 3 and 14 post-infection. (B) Venn diagram showing number of differentially expressed CMGs on day 14 post-infection. (C) GO enrichment analysis of differentially expressed CMGs on day 14 post-infection. (D) Representative images of Filipin III staining in BMDMs infected with S. aureus for 12 hours. Scale bar was 5µm. (E) Fluorescence intensity result of Filipin III staining. (F) Schematic of the isolation of bone marrow and bone marrow supernatant. Images were created with BioRender.com. (G) Cholesterol content in CD11b+F4/80+ macrophages and supernatant from S. aureus osteomyelitis mice and controls. n = 4/group. Data are presented as mean ± SEM. Two-tailed Student’s t-test was used. *P < 0.05.