Fig 1: Analysis of T and B cells in total lung cells of OVA-treated IL-3-/- mice(A andB) Flow cytometry analysis of CD19+ CD45R+ B cells (n = 5) (a) and CD103+ cDCs (n = 4/4/5/5) (b) cells in total lung cells isolated from BALB/c WT and IL3-/- mice with and without asthma.(C) ELISA analysis of the IL-4 level in cell culture supernatants obtained from total lung cells of mice with and without asthma (n = 14/14/15/15).(D and E) CD4+ and CD8+ T cells (d) and Foxp3+ CD25+ CD4+ Treg (e) (n = 10 (CD4+ cells), n = 5 (CD8+, Treg)). A representative dot plot is shown for each group. Data are presented as means ± SEMs. two-way ANOVA was used to calculate statistical significance. *p = 0.05; **p = 0.01, ***p = 0.001, ****p = 0.0001.