Fig 2: Bnc1 mutation induces follicular atresia through nonapoptotic cell death.a Ovaries were obtained from Bnc1+/+ (n = 3) and Bnc1tr/tr mice (n = 3) at 16 weeks old for western blotting (WB). The expression levels of P53, Bcl2, Bax, PARP, Caspase3 and cleaved-caspase3 are shown (p value = 0.6585 for P53, p value = 0.0457 for Bcl2, p value = 0.3090 for Bax, p value = 0.3467 for BAX/BCL2, p value = 0.0624 for Caspase3, p value = 0.4892 for cleaved-Caspase3/Caspase3 and p value = 0.4162 for PARP). b GV oocytes were obtained from Bnc1+/+ (n = 3) and Bnc1tr/tr (n = 3) mice at 4 weeks old for real-time PCR. The mRNA expression of Bax and Bcl2 is shown (p value = 0.7446 for Bax, p value = 0.0241 for Bcl2 and p value = 0.0247 for Bax/Bcl2). c GV oocytes obtained from Bnc1+/+ and Bnc1tr/tr mice at 4 weeks old were used for detection of early apoptosis (3 independent experiments with total oocyte numbers >30 oocytes). d GV oocytes obtained from Bnc1+/+ and Bnc1tr/tr mice at 4 weeks old were used for detection of Caspase3 (CAS3) and the DNA damage marker ?-H2AX (3 independent experiments with total oocyte numbers >30 oocytes). The error bars indicate the mean values ± SDs, unpaired t test, two-tailed, *p value < 0.05, **p value < 0.01 and ***p value < 0.001. Source data are provided as a Source Data file.

Fig 3: Identification and molecular docking verification of PBP anti-lung cancer core targets. (a) PPI network diagram obtained by STRING database analysis (interaction score > 0.9); (b) Visualizing the PPI network was completed using Cytoscape 3.9.1; (c) The docking mode of CASP3- osmundacetone, PARP-osmundacetone, TP53-osmundacetone, CASP3-hispidin, PARP-hispidin and TP53-hispidin.