Fig 1: (a) Mechanism of antiaging/atresia of Met via the calcium ion (Ca2+) transport signaling pathway in the H2O2-induced ASWFs. A: the effect of Met on the expression of GRP75, Cyt c, and MFN2 with IF and IHC analyses. Scale bar: 50 μm. B: Western blot and grey analysis of IP3R, caspase3, GRP75, CAMKII, MFN2, and Cyt c expression in ASWFs. C: effect of Met on expression of Ca2+ transport-related genes. (b) Mechanism of antiaging/atresia of Met via the insulin signaling pathway. A: the effect of Met on expression of PI3K (red), BAD (green), and DAPI (blue) with IF. Scale bar: 50 μm. B: Western blot and grey analysis of p-AKT/AKT and p-GSK-3β/GSK-3β expression in ASWFs. C: effect of Met on expression of PI3K, BCL2, and GLUT4 mRNAs in ASWFs. (c) Mechanism of antiaging/atresia of Met via the lipid metabolism signaling pathway. A: Western blot and grey analysis of PPAR-γ, occludin, and VLDLR expression in ASWFs. B: qRT-PCR analysis of PPAR-γ, occludin, and VLDLR expression in ASWFs. Values represent the means ± SEM of three replicates in each group. Different lowercase letters indicate significant difference (P < 0.05).