Fig 1: Binding of RXRa correlates with chromatin openness and gene expression.a Occupancy of RXRa on MI-DARs. The percentage of RXRa-bound sites and RXRa motif enrichment of MI-DARs (left). P value was calculated by HOMER, using the binomial distribution. ATAC-seq signals in DARs (right). b ATAC-seq signals on RXRabound and RXRaunbound sites. c Pearson’s correlation coefficients of RXRa ChIP-seq signals and ATAC-seq signals on RXRabound regions. Statistical significance was determined by the Correlation test. P < 2.2 × 10-16. d Gene expression levels of RXRabound and RXRaunbound site-associated genes. Box plots represent median values, 25%, and 75% quantiles. Whiskers extend to 1.5 times the interquartile range. Statistical significance was determined by the two-sided Mann–Whitney U test. P = 1.76 × 10-13 (RXRabound: n = 1194 genes, RXRaunbound: n = 2870 genes). e qRT-PCR analysis of Rxra mRNA in kidney tissues from Sham and AKI mice. From left to right: **P = 0.0017, NS P = 0.8569, *P = 0.0202, respectively, by two-tailed unpaired Student’s t-test. NS not significant. n = 5 biologically independent samples. f Immunohistochemistry staining of RXRa in kidney tissues from Sham and AKI mice. From left to right: ****P < 0.0001, NS P = 0.1729, ***P = 0.0005, respectively, by two-tailed unpaired Student’s t-test. NS not significant. n = 5 biologically independent samples. Scale bars, 50 µm. g RXRa ChIP-seq and ATAC-seq signals in the SI group. h Volcano plot showing gene expression of RXRa-activated renoprotective genes. Blue or red represents a decrease and increase, respectively. Statistical analysis was calculated by Metascape. i Representative IGV tracks showing RXRa ChIP-seq, ATAC-seq, and RNA-seq signals of these 319 genes. The signal strength is denoted by color intensities (a, b, g). Data are represented as means ± SEM (e, f). Source data are provided as a Source Data file.