Fig 2: NPR1 and SGK1 expression levels. (A) Analysis of NPR1 and SGK1 expression levels in the heart tissue of WT, Npr1+/-, WT + DEX and Npr1+/- + DEX mice (n=4/group) using immunofluorescence (magnification, x100; scale bar, 100 µm). (B) Analysis of NPR1 and SGK1 expression levels in the heart tissues of WT, Npr1+/-, WT + DEX and DEX + Npr1+/- mice using western blotting. (C) Relative expression of SGK1 using western blotting. (D) Relative expression levels of NPR1 using western blotting. *P<0.05. Npr1, natriuretic peptide receptor 1; WT, wild-type; DEX, dexamethasone; SGK1, serum/dexamethasone regulated kinase 1.