Fig 1: GSPs prevent oxidative stress-induced GC injury via preferentially inhibiting autophagic death. (A) Cell viability was determined using the CCK-8 assay. Z-VAD-FMK (ZVF, a well-known pan-caspase inhibitor, 50 μM). (B) GCs pretreated with 10 μM GSPs for 24 h were then rinsed in PBS and exposed to 100 μM H2O2 for 2 h. To block the autophagic flux, 3-MA (10 mM) was added before H2O2 exposure. Western blotting showed expression levels of LC3B and SQSTM1. (C) Quantification of immunoblot signals for SQSTM1 and LC3B accumulation. (D) GCs pretreated with 10 μM GSPs for 24 h were then rinsed in PBS and exposed to 100 μM H2O2 for 2 h. To block the apoptosis, ZVF (50 μM) was added before H2O2 exposure. (E) The protein levels of Caspase 3 and Bcl2A1 were evaluated. Bars with different superscripts are statistically different (p < 0.05). Values are the means ± SEM (n = 3).