Fig 2: CK2 overexpression reduced cell inflammation, apoptosis and oxidative stress through changing NR2B phosphorylation level and NR2B-PSD95 complex. pc-NR2B was transfected into Hemin + pc-CK2 treated neurons and astrocytes. (A) Expression of NR2B, NR2B phosphorylation sites S1480, S1303, and Tyr1472 were determined in cells using Western blot. (B) Representative gel images and quantification of co-immunoprecipitation (co-IP) show the level of NR2B-PSD95 complex in cells. (C) Expressions of NR2B in cells were detected using RT-qPCR. (D) NR2B expression was detected using Western blot. (E) Levels of TNF-a and IL-6 in astrocytes were detected using ELISA. (F) Cell apoptosis was measured using TUNEL assay. (G) Content of ROS was determined by fluorescence probe DCFH-DA. The cell experiment was repeated three times independently. Data were expressed as mean ± standard deviation. **p < 0.01, ***p < 0.001, ****p < 0.0001.