Fig 1: Isorhamnetin prevented ZEA-induced steroid secretion disorder in a PI3K/Akt-dependent manner. Ovarian GC were cultured with isorhamnetin (20 µmol/L) for 2 h with or without LY294002 (20 µmol/L), before treatment with ZEA (60 µmol/L) for 24 h. (A) Western blot analysis of FSHR and CYP19A1 in ovarian GC treated with ZEA or isorhamnetin. (B to C) The average optical density value of FSHR and CYP19A1 normalized to ß-actin. (D) The content of estrogen (E2) in ovarian GC treated with ZEA, isorhamnetin or LY294002. (E to H) The mRNA expression of FSHR, ER2, CYP19A1 and ER1 were tested by an RT-PCR assay. Data are expressed as means ± SEM. Different letters mean significant differences (P < 0.05). ZEA = zearalenone; PI3K = phosphatidylinositol 3 kinase; Akt = protein kinase B; GC = granulosa cell; FSHR = follicle-stimulating hormone receptor; CYP19A1 = cytochrome P450 family 19 subfamily a member 1.