Fig 1: Immunohistochemistry stained localization of ST6GAL1, GNAQ, ADFP, and PCNA in UVJ of egg layer breeders with high and low sperm storage capacity. (A-B) ST6GAL1 proteins were localized at epithelial cytoplasm in (A) high-SS and (B) low-SS hen UVJ. (C-D) The positive signals of GANQ antibody were localized at epithelial cell membranes in (C) high-SS and (D) low-SS hen UVJ. (E-F) The strong positive signals of ADFP antibody staining were converged on SST in (E) high-SS and (F) low-SS hen UVJ. (G-H) Positive PCNA signals appeared in all epithelial cells in (G) high-SS and (H) low-SS hen UVJ. (I) The negative control without incubating the primary antibody in immunohistochemistry. Abbreviations: SS, sperm storage; SST, sperm storage tubules; UVJ, uterovaginal junction; A-F and I, Bar, 100 µm. G and H, Bar, 200 µm.