Fig 2: Cell localization and expression of FDX1, GLS, and SLC31A1 in AS single-cell data samples. a Classification of cell populations and location of specific genes in datasets GSE155514. b-d Dot plots (in red) showing the expression of three hub genes on the t-SNE map: FDX1 (b), GLS (c), and SLC31A1 (d). e Dot plot showing the expression of three hub genes in each cluster. Deeper and larger dots indicate higher expression levels

Fig 3: The diagnostic value of 3 Hub DE-CRGs in AS. a-d The diagnostic power of FDX1, GLS, and SLC31AL in atherosclerosis by ROC curve and intergroup overall inspection of ROC analysis in datasets GSE43292 (a), GSE132651 (b), GSE28829 (c), and GSE120521 (d)

Fig 4: Immunohistochemistry for FDX1, GLS and SLC31A1. a-c Immunohistochemical staining of FDX1 (a), GLS (b), and SLC31A1 (c) in human normal coronary arterial tissues sections (Control) and AS tissues (Plaque), and mean density of FDX1 + (% area), GLS + (% area), SLC31A1 + (% area). Scale bar: 200 µm and 50 µm. ***P < 0.001, **P < 0.01 and *P < 0.05 vs. control group. n = 8