Fig 1: RepSox can induce the transformation of EGCs into neurons without reprogramming of EGCs to neural stem cells in vitro.a TUJ1 immunofluorescence staining was performed on Days 4, 8 and 14 of culture to reveal the morphological changes of the cells. Scale bar = 50 µm. b Immunofluorescence staining analysis of S100ß and TUJ1 on Days 8 and 30 after RepSox induction, respectively. Scale bar = 50 µm. c Statistics of (b) (mean ± SEM, n = 3). d, e Real-time PCR analysis of mRNA expression in cells during RepSox induction. Data were shown as the fold change versus Day 0 (mean ± SEM, n = 3, ***P < 0.001 vs. Day 0; ###P < 0.001 vs. its corresponding maximum expression). f Immunofluorescence staining was performed to analyse the expression of the neural stem cell markers P75, EDNRB and Sox2 in EGCs 0, 4 and 8 days after RepSox treatment. Scale bar = 50 µm. g Statistical analysis of (f).