Fig 2: Theophylline increases Sox10, Krox20, and P0 expression and eEF1A1 deacetylation.a HDAC2 (12dpl: n = 3, p = 0.025883, 14dpl: n = 5, p = 0.011697), Sox10 (12dpl: n = 3, p = 0.031422, 14dpl: n = 3–4, p = 0.032671), Krox20 (12dpl: n = 3, p = 0.030486, 14dpl and 30dpl: n = 7, p = 0.033493 and 0.022041) and P0 (12dpl: n = 3, p = 0.022239, 14dpl: n = 4-5, p = 0.019084, 30dpl: n = 6, p = 0.0456) Western blots at 12, 14 and 30dpl in lysates of crushed (Cr) or contralateral (Co) sciatic nerves of mice treated at 10dpl with theophylline (Theo) or vehicle (Veh.) for 2 days (12dpl) or 4 days (14 and 30dpl), and quantification normalized to GAPDH and Co. b, c EEF1A1 IP and Ac-eEF1A Western blot (b) and Ac-eEF1A and S100 (SC marker) co-immunofluorescence and DAPI labeling (c) at 12dpl in Cr (c) or in Cr and Co (b) 20 h after one theophylline or vehicle injection, and (b) Ac-eEF1A1 quantification normalized to GAPDH input and Co in theophylline- compared to vehicle-treated groups. N = 3 animals per group (b, c), p = 0.000597 (b). Representative images are shown. d–f GFP (d) and/or Sox10 (d–f) chromatin immunoprecipitation at 12dpl on Krox20 MSE or P0 intron 1 in (d) Cr and fold enrichment normalized to Sox10 input compared to GFP (n = 3 animals, Sox10 and GFP IPs on the same nerve lysate, pKrox20-MSE = 0.032616, pP0-intron-1 = 0.027702) or in Cr (e) collected 20 h after theophylline (Theo) or vehicle (Veh.) treatment or (f) after mocetinostat (Mocet.) or Veh. treatment for 2 days. e N = 5–6 animals per group, pKrox20-MSE = 0.030466, pP0-intron-1 = 0.04003. f N = 3 animals per group, pKrox20-MSE = 0.037396, pP0-intron-1 = 0.023672. g Sox10, P0, HDAC2, and GAPDH Western blots in lysates of P4 DhhCre;Hdac1fl/fl;Hdac2fl/fl dKO and control littermate sciatic nerves treated at P1 with Theo or Veh. for 3 days, and quantification normalized to GAPDH in Theo compared to Veh. N = 6 animals per group, pSox10 = 0.04567, pP0 = 0.033358, pHDAC2 = 0.02891. Dashed lines: samples loaded on the same gel but not on consecutive lanes. Data are presented as mean values ± SEM. Paired (a: HDAC2 and Sox10-12dpl, Krox20-14dpl-30dpl; b, d, e, and f: P0-intron-1; g) or unpaired one-tailed (gray asterisks) or two-tailed (black asterisks) Student’s t-tests. Scale bar: 20 µm (c). Source data are provided as a Source Data file.