Fig 1: Effects of IW-1973 on WAT. (A) Body weight progression during the 16 weeks of treatment of the mice receiving chow diet (n = 5), HFD diet (n = 10), HFD plus sGC stimulator IW-1973 at 1 mg·kg-1 (n = 10) or HFD plus IW-1973 at 3 mg·kg-1 (n = 10). (B) WAT and BAT weight to body weight ratios. (C) Representative photomicrographs (200× magnification) of WAT sections stained with H&E and a specific F4/80 antibody. (D) Histomorphometric analysis of the area immunostained with the specific F4/80 macrophage antibody. (E) Adipocyte area assessed by ImageJ morphometric analysis as described in the Methods section. (F) p-HSL and ß-actin determined in WAT by SDS-PAGE Western blot. (G) ATG7, ATG12-5, p62, LC3-I/ II and ß-actin determined in WAT by SDS-PAGE Western blot analysis. The densitometric analyses of LC3-II/LC3-I ratio and p62 are shown on the right. (H) ATG7, ATG12-5, p62, LC3-I/LC3-II and ß-actin in differentiated 3T3 adipocytes incubated with vehicle (Veh) or increasing concentrations of IW-1973 (1, 3 and 10 µM). Results are expressed as mean ± SEM. *P < 0.05 for HFD versus CHOW. Blot data subjected to statistical analysis were from n = 5 experiments. Scale bar = 50 µm.