Fig 1: Developmental patterning is unaffected in Arpc5 knockdown(A) Z sections of control (sqh-GFP[29B]; Dll-Gal4) and Arpc5 RNAi (sqh-GFP[29B]; Dll-Gal4; UAS-Arpc5RNAi) leg discs stained with phalloidin (white) and showing Z projection of anti-Deadpan (magenta).(B) Z sections of control (rpr-lacZ; Dll-Gal4) or Arpc5 RNAi (rpr-lacZ; Dll-Gal4; UAS-Arpc5RNAi) leg disks stained with phalloidin (white) and showing a Z projection of rpr-lacZ (yellow).(A and B) Yellow, blue, and green arrowheads indicate predicted fold, real fold, and perfect match between them, respectively. Please note that both Dpn and Rpr-lacZ are also expressed in some neurons, indicated by asterisks. Cartoons on the right recapitulate the location of Notch signaling (purple) and pro-apoptotic genes expression (yellow) in control and Arpc5 RNAi conditions.(C and D) Dot plots showing the angle of the stripes of expression of Deadpan (C) or reaper (D) in the folds relative to the proximal-distal axis in control (rpr-lacZ; Dll-Gal4) and Arpc5 RNAi (rpr-lacZ; Dll-Gal4; UAS-Arpc5RNAi) leg discs (n = 13 and 11 respectively). An F test of equality of variances was used. ns, not significant. Black lines represent the mean ± SD.(E and F) 3D reconstructions of control (E) and Arpc5 RNAi (F) leg discs showing the expression of Deadpan (magenta, arrows). Yellow and blue domain highlight the predicted and real folds, respectively. Asterisks point out Dpn-expressing neurons.(G) Average number of dying cells inside or outside the predicted distal-most tarsal fold in control and Arpc5 RNAi leg discs (n = 20 and 28, respectively). Bar graphs indicate the mean ± SEM. Cartoons on the left recapitulate the regions inside (yellow) and outside (brown) of the predicted fold.(H and I) Sagittal views showing apoptotic myosin cables (H) and dot plots showing the initial velocity of apoptotic cable recoil after laser microdissection (I) in control (sqh-GFP[29B]; Dll-Gal4) and Arpc5 RNAi (sqh-GFP[29B]; Dll-Gal4; UAS-Arpc5RNAi) leg discs (n = 6 and 10, respectively). Black line indicates the median. In (G) and (I), the statistical significance was calculated using Mann-Whitney U test. ns, not significant. Scale bars: 20 µm in (A), (B), (E), and (F); 2 µm in (H).Related to Figure S2; Videos S1, S2, and S3.