Fig 1: Impact of METH and/or mIL1T on IL-1ß, IL-6, IL-1RacP, and IL-1R1 expression. Mice were administered with METH and/or mIL1T as described in the Materials and Methods section, and hippocampal protein levels of IL-1ß (A) and IL-6 (B) were measured by ELISA. The results were analyzed by two-way-ANOVA followed by Tukey post hoc test; mean ± S.E. The positive Pearson’s correlation was observed among IL-1ß and IL-6 levels in the hippocampi (C) and cortices (D) of examined mice. In addition, hippocampal protein expression of IL-1RacP (E, F) and IL-1R1 (E, G) were evaluated by immunoblotting. The blots were normalized to ßactin levels and analyzed by the Kruskal–Wallis test with Dunn’s post hoc test; the results are median ± min to max. (E) Representative images, and (F and G) quantitative data from these experiments. All measurements were performed 24 h after the last METH injection (n = 3–4; *p < 0.05, **p < 0.01).