Fig 1: BAP treated H1 ESC were transiently transfected with VTCN1 siRNA or a scrambled siRNA control for 3d. (A) The effects of VTCN1 KD on HLA-A, HLA-B and HLA-C transcription were assessed by RT-PCR. Data represents means ± SD of three independent experiments; statistically significant comparisons to scrambled control siRNA are indicated by asterisks (p<0.05). (B) Protein was collected on BAP d6 (transfection d3) and the expression of HLA-A, HLA-B and HLA-C were assessed by western blotting (C) Immunofluorescence microscopy was performed to assess the expression of HLA-A, HLA-B and HLA-C on BAP d6 (transfection d3) (Scale bar, 100 µm). (D) Western blotting analysis of HLA-A protein expression in BAP treated H1 cells or VTCN1 KO cells on BAP d4, d6 and d8. (E) ISG15, OAS1 and MX1 mRNA levels for BAP treated H1 cells (WT) and VTCN1 KO cells were assessed by qPCR on BAP d6.